Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22230-60] to Cripto1/CRIPTO
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-Cripto1/CRIPTO antibody [EPR22230-60]
See all Cripto1/CRIPTO primary antibodies
DescriptionRabbit monoclonal [EPR22230-60] to Cripto1/CRIPTO
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human Cripto1/CRIPTO aa 1-150. The exact sequence is proprietary.
Database link: P13385
- WB: NCCIT whole cell lysate. IP: NCCIT whole cell lysate.
This product was previously labelled as Cripto1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab229016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 17, 21 kDa (predicted molecular weight: 21 kDa).|
FunctionCould play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Tissue specificityPreferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
Sequence similaritiesContains 1 EGF-like domain.
Cellular localizationCell membrane.
- Information by UniProt
- CR antibody
- CRGF antibody
- Cripto 1 antibody
Cripto1/CRIPTO was immunoprecipitated from 0.35 mg of NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate with ab229016 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229016 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: NCCIT whole cell lysate 10 µg (Input).
Lane 2: ab229016 IP in NCCIT whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229016 in NCCIT whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 2 minutes.
Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016) at 1/1000 dilution + NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
The double bands observed are consistent with what has been described in the literature. The lower band represents the immature form of Cripto1/CRIPTO. The upper band is likely to be the processed glycosylated form (PMID: 27881714 and 20568225).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229016 has not yet been referenced specifically in any publications.