Recombinant
RabMAb

Recombinant Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)

Overview

  • Product name

    Anti-Cripto1/CRIPTO antibody [EPR22230-60]
    See all Cripto1/CRIPTO primary antibodies
  • Description

    Rabbit monoclonal [EPR22230-60] to Cripto1/CRIPTO
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Cripto1/CRIPTO aa 1-150. The exact sequence is proprietary.
    Database link: P13385

  • Positive control

    • WB: NCCIT whole cell lysate. IP: NCCIT whole cell lysate.
  • General notes

     This product was previously labelled as Cripto1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab229016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 17, 21 kDa (predicted molecular weight: 21 kDa).
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
    • Tissue specificity

      Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
    • Sequence similarities

      Contains 1 EGF-like domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CR antibody
      • CRGF antibody
      • Cripto 1 antibody
      • Cripto 1 growth factor antibody
      • cripto antibody
      • Cripto-1 growth factor antibody
      • Cripto1 growth factor antibody
      • Epidermal growth factor like cripto protein CR1 antibody
      • Epidermal growth factor-like cripto protein CR1 antibody
      • TDGF 1 antibody
      • TDGF1 antibody
      • TDGF1_HUMAN antibody
      • Teratocarcinoma derived growth factor 1 antibody
      • Teratocarcinoma-derived growth factor 1 antibody
      see all

    Images

    • Cripto1/CRIPTO was immunoprecipitated from 0.35 mg of NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate with ab229016 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229016 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: NCCIT whole cell lysate 10 µg (Input). 

      Lane 2: ab229016 IP in NCCIT whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229016 in NCCIT whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 2 minutes.

    • Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016) at 1/1000 dilution + NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 21 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      The double bands observed are consistent with what has been described in the literature. The lower band represents the immature form of Cripto1/CRIPTO. The upper band is likely to be the processed glycosylated form (PMID: 27881714 and 20568225).

    References

    ab229016 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab229016.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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