Recombinant Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016)


  • Product name

    Anti-Cripto1/CRIPTO antibody [EPR22230-60]
    See all Cripto1/CRIPTO primary antibodies
  • Description

    Rabbit monoclonal [EPR22230-60] to Cripto1/CRIPTO
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Cripto1/CRIPTO aa 1-150. The exact sequence is proprietary.
    Database link: P13385

  • Positive control

    • WB: NCCIT whole cell lysate. IP: NCCIT whole cell lysate.
  • General notes

     This product was previously labelled as Cripto1


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab229016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 17, 21 kDa (predicted molecular weight: 21 kDa).
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
    • Tissue specificity

      Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
    • Sequence similarities

      Contains 1 EGF-like domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CR antibody
      • CRGF antibody
      • Cripto 1 antibody
      • Cripto 1 growth factor antibody
      • cripto antibody
      • Cripto-1 growth factor antibody
      • Cripto1 growth factor antibody
      • Epidermal growth factor like cripto protein CR1 antibody
      • Epidermal growth factor-like cripto protein CR1 antibody
      • TDGF 1 antibody
      • TDGF1 antibody
      • TDGF1_HUMAN antibody
      • Teratocarcinoma derived growth factor 1 antibody
      • Teratocarcinoma-derived growth factor 1 antibody
      see all


    • Cripto1/CRIPTO was immunoprecipitated from 0.35 mg of NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate with ab229016 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229016 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: NCCIT whole cell lysate 10 µg (Input). 

      Lane 2: ab229016 IP in NCCIT whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229016 in NCCIT whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 2 minutes.

    • Anti-Cripto1/CRIPTO antibody [EPR22230-60] (ab229016) at 1/1000 dilution + NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate at 20 µg

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 21 kDa

      Blocking/Dilution buffer: 5% NFDM/TBST.

      The double bands observed are consistent with what has been described in the literature. The lower band represents the immature form of Cripto1/CRIPTO. The upper band is likely to be the processed glycosylated form (PMID: 27881714 and 20568225).


    ab229016 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab229016.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact

    Sign up