Recombinant Anti-Cripto1/CRIPTO antibody [EPR22230-60] - BSA and Azide free (ab244553)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22230-60] to Cripto1/CRIPTO - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cripto1/CRIPTO antibody [EPR22230-60] - BSA and Azide free
See all Cripto1/CRIPTO primary antibodies -
Description
Rabbit monoclonal [EPR22230-60] to Cripto1/CRIPTO - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: NCCIT whole cell lysate.
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General notes
ab244553 is the carrier-free version of ab229016.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22230-60 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 17, 21 kDa (predicted molecular weight: 21 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 17, 21 kDa (predicted molecular weight: 21 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. -
Tissue specificity
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. -
Sequence similarities
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6997 Human
- Omim: 187395 Human
- SwissProt: P13385 Human
- Unigene: 385870 Human
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Alternative names
- CR antibody
- CRGF antibody
- Cripto 1 antibody
see all
Images
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Cripto1/CRIPTO was immunoprecipitated from 0.35 mg of NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate with ab229016 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229016 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: NCCIT whole cell lysate 10 µg (Input).
Lane 2: ab229016 IP in NCCIT whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229016 in NCCIT whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 2 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229016).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab244553 has not yet been referenced specifically in any publications.