Product nameAnti-Cripto1/CRIPTO antibody [EPR22230-60] - BSA and Azide free
See all Cripto1/CRIPTO primary antibodies
DescriptionRabbit monoclonal [EPR22230-60] to Cripto1/CRIPTO - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human Cripto1/CRIPTO aa 1-150. The exact sequence is proprietary.
Database link: P13385
- IP: NCCIT whole cell lysate.
ab244553 is the carrier-free version of ab229016 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab244553 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Cripto1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab244553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 17, 21 kDa (predicted molecular weight: 21 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionCould play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Tissue specificityPreferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
Sequence similaritiesContains 1 EGF-like domain.
Cellular localizationCell membrane.
- Information by UniProt
- CR antibody
- CRGF antibody
- Cripto 1 antibody
Cripto1/CRIPTO was immunoprecipitated from 0.35 mg of NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate with ab229016 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229016 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: NCCIT whole cell lysate 10 µg (Input).
Lane 2: ab229016 IP in NCCIT whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 2 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229016).
ab244553 has not yet been referenced specifically in any publications.