Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626)

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Western blot - Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626)

    Key features and details

    • Mouse monoclonal [mAbcam60626] to Cripto1/CRIPTO
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-Cripto1/CRIPTO antibody [mAbcam60626]
      See all Cripto1/CRIPTO primary antibodies

    • Description

      Mouse monoclonal [mAbcam60626] to Cripto1/CRIPTO

    • Host species

      Mouse

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Recombinant fragment
      Predicted to work with: Mouse, Cow, Human

    • Immunogen

      Synthetic peptide corresponding to Human Cripto1/CRIPTO aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab23310)

    • Positive control

      • This antibody gave a positive signal in Cripto1/CRIPTO (Human) Recombinant Protein.

    • General notes

      This antibody clone is manufactured by Abcam.

      If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

       This product was previously labelled as Cripto1

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab60626 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Notes
  • Application notes
    WB: Use at a concentration of 10 µg/ml. Detects a band of approximately 21 kDa using a Cripto1 Recombinant Protein (predicted molecular weight: 16-20 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

    • Tissue specificity

      Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.

    • Sequence similarities

      Contains 1 EGF-like domain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CR antibody
        • CRGF antibody
        • Cripto 1 antibody
        • Cripto 1 growth factor antibody
        • cripto antibody
        • Cripto-1 growth factor antibody
        • Cripto1 growth factor antibody
        • Epidermal growth factor like cripto protein CR1 antibody
        • Epidermal growth factor-like cripto protein CR1 antibody
        • TDGF 1 antibody
        • TDGF1 antibody
        • TDGF1_HUMAN antibody
        • Teratocarcinoma derived growth factor 1 antibody
        • Teratocarcinoma-derived growth factor 1 antibody
        see all

      Images

      • Western blot - Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626)
        Western blot - Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626)
        Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626) at 10 µg/ml + Cripto1/CRIPTO Recombinant Protein at 0.1 µg

        Secondary
        Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Performed under reducing conditions.

        Predicted band size: 16-20 kDa
        Observed band size: 21 kDa why is the actual band size different from the predicted?

      References (0)

      Publishing research using ab60626? Please let us know so that we can cite the reference in this datasheet.

      ab60626 has not yet been referenced specifically in any publications.

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