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Stem Cells Lineage Markers Ectoderm
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Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626)

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Western blot - Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626)

    Key features and details

    • Mouse monoclonal [mAbcam60626] to Cripto1/CRIPTO
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG2a

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    Protein
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    Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)

    View more associated products

    Overview

    • Product name

      Anti-Cripto1/CRIPTO antibody [mAbcam60626]
      See all Cripto1/CRIPTO primary antibodies
    • Description

      Mouse monoclonal [mAbcam60626] to Cripto1/CRIPTO
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Recombinant fragment
      Predicted to work with: Mouse, Cow, Human
    • Immunogen

      Synthetic peptide corresponding to Human Cripto1/CRIPTO aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab23310)

    • Positive control

      • This antibody gave a positive signal in Cripto1/CRIPTO (Human) Recombinant Protein.
    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 6.97% L-Arginine, PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      mAbcam60626
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG2a
    • Research areas

      • Stem Cells
      • Lineage Markers
      • Ectoderm
      • Stem Cells
      • Embryonic Stem Cells
      • Secreted
      • Developmental Biology
      • Embryogenesis
      • Morphogens
      • Developmental Biology
      • Lineage specification
      • Ectoderm

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Positive Controls

      • Recombinant human Cripto1/CRIPTO protein (ab84064)
    • Recombinant Protein

      • Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab60626 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Notes
    Application notes
    WB: Use at a concentration of 10 µg/ml. Detects a band of approximately 21 kDa using a Cripto1 Recombinant Protein (predicted molecular weight: 16-20 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    • Function

      Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
    • Tissue specificity

      Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
    • Sequence similarities

      Contains 1 EGF-like domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P13385 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 784029 Cow
      • Entrez Gene: 6997 Human
      • Entrez Gene: 21667 Mouse
      • Omim: 187395 Human
      • SwissProt: P13385 Human
      • SwissProt: P51865 Mouse
      • Unigene: 385870 Human
      • Alternative names

        • CR antibody
        • CRGF antibody
        • Cripto 1 antibody
        • Cripto 1 growth factor antibody
        • cripto antibody
        • Cripto-1 growth factor antibody
        • Cripto1 growth factor antibody
        • Epidermal growth factor like cripto protein CR1 antibody
        • Epidermal growth factor-like cripto protein CR1 antibody
        • TDGF 1 antibody
        • TDGF1 antibody
        • TDGF1_HUMAN antibody
        • Teratocarcinoma derived growth factor 1 antibody
        • Teratocarcinoma-derived growth factor 1 antibody
        see all

      Images

      • Western blot - Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626)
        Western blot - Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626)
        Anti-Cripto1/CRIPTO antibody [mAbcam60626] (ab60626) at 10 µg/ml + Cripto1/CRIPTO Recombinant Protein at 0.1 µg

        Secondary
        Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Performed under reducing conditions.

        Predicted band size: 16-20 kDa
        Observed band size: 21 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab60626? Please let us know so that we can cite the reference in this datasheet.

      ab60626 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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