Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18991] to CRISPR-Cas9 (HRP)
- Suitable for: WB
- Reacts with: Streptococcus pyogenes
- Conjugation: HRP
Product nameAnti-CRISPR-Cas9 antibody [EPR18991] (HRP)
See all CRISPR-Cas9 primary antibodies
DescriptionRabbit monoclonal [EPR18991] to CRISPR-Cas9 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Streptococcus pyogenes
Recombinant fragment within Streptococcus pyogenes CRISPR-Cas9 aa 800-1000. The exact sequence is proprietary. (Serotype M1).
Database link: Q99ZW2
- WB: HEK-293T transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab215345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/8000. Detects a band of approximately 158 kDa (predicted molecular weight: 158 kDa).|
Relevance[FUNCTION] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
- Cas9 antibody
- CRISPR-associated endonuclease Cas9/Csn1 antibody
- CRISPR-Cas9/Csn1 antibody
All lanes : Anti-CRISPR-Cas9 antibody [EPR18991] (HRP) (ab215345) at 1/8000 dilution
Lane 1 : HEK-293T (Human embryonic kidney epithelial cell) transfected with vector control (pcDNA3.1(+)-EGFP-Myc), whole cell lysate
Lane 2 : HEK-293T transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
Lane 3 : HEK-293T transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-Myc tag, whole cell lysate
Lane 4 : HEK-293T transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-Myc tag, whole cell lysate
Lane 5 : HEK-293T transfected vector control with GFP-Myc tag, whole cell lysate
Lane 6 : HEK-293T transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)) with Myc-His tag, whole cell lysate
Lane 7 : HEK-293T transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag, whole cell lysate
Lane 8 : HEK-293T transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)) with Myc-His tag, whole cell lysate
Lane 9 : HEK-293T transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag, whole cell lysate
Predicted band size: 158 kDa
Observed band size: 184 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab215345 has not yet been referenced specifically in any publications.