Recombinant
RabMAb

Recombinant Anti-CRISPR-Cas9 antibody [EPR18991] (HRP) (ab215345)

Overview

  • Product name

    Anti-CRISPR-Cas9 antibody [EPR18991] (HRP)
    See all CRISPR-Cas9 primary antibodies
  • Description

    Rabbit monoclonal [EPR18991] to CRISPR-Cas9 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Streptococcus pyogenes
  • Immunogen

    Recombinant fragment within Streptococcus pyogenes CRISPR-Cas9 aa 800-1000. The exact sequence is proprietary. (Serotype M1).
    Database link: Q99ZW2

  • Positive control

    • WB: HEK-293T transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18991
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab215345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000. Detects a band of approximately 158 kDa (predicted molecular weight: 158 kDa).

Target

  • Relevance

    [FUNCTION] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
  • Database links

    • Alternative names

      • Cas9 antibody
      • CRISPR-associated endonuclease Cas9/Csn1 antibody
      • CRISPR-Cas9/Csn1 antibody
      • csn1 antibody
      • SpyCas9 antibody
      see all

    Images

    • All lanes : Anti-CRISPR-Cas9 antibody [EPR18991] (HRP) (ab215345) at 1/8000 dilution

      Lane 1 : HEK-293T (Human embryonic kidney epithelial cell) transfected with vector control (pcDNA3.1(+)-EGFP-Myc), whole cell lysate
      Lane 2 : HEK-293T transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
      Lane 3 : HEK-293T transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-Myc tag, whole cell lysate
      Lane 4 : HEK-293T transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-Myc tag, whole cell lysate
      Lane 5 : HEK-293T transfected vector control with GFP-Myc tag, whole cell lysate
      Lane 6 : HEK-293T transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)) with Myc-His tag, whole cell lysate
      Lane 7 : HEK-293T transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag, whole cell lysate
      Lane 8 : HEK-293T transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)) with Myc-His tag, whole cell lysate
      Lane 9 : HEK-293T transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag, whole cell lysate

      Predicted band size: 158 kDa
      Observed band size: 184 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes

    References

    ab215345 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab215345.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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