Recombinant
RabMAb

Recombinant Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)

Rabbit recombinant monoclonal CRISPR-Cas9 antibody [EPR19619-92]. Validated in WB and tested in Streptococcus pyogenes. Cited in 1 publication(s). Immunogen corresponding to recombinant fragment.

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Overview

  • Product name

    Anti-CRISPR-Cas9 antibody [EPR19619-92]
    See all CRISPR-Cas9 primary antibodies

  • Description

    Rabbit monoclonal [EPR19619-92] to CRISPR-Cas9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Streptococcus pyogenes, Streptococcus thermophilus

  • Immunogen

    Recombinant fragment within CRISPR-Cas9 aa 150-600. The exact sequence is proprietary. Streptococcus pyogenes serotype M1 and Streptococcus thermophilus (strain ATCC BAA-491/ LMD-9).
    Database link: G3ECR1

  • Positive control

    • WB: HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-tag; HEK-293 whole cell lysate transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491/LMD-9)) with His-tag; HEK-293 whole cell lysate transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A) with His-tag. IHC-P: 293T cells transfected with Streptococcus thermophilus CRISPR-Cas9 (pcDNA3.1(+)-Myc-His)

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19619-92

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab210752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 164 kDa (predicted molecular weight: 164 kDa).

Target

  • Relevance

    [FUNCTION] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

  • Database links

    • Alternative names

      • Cas9 antibody
      • CRISPR-associated endonuclease Cas9/Csn1 antibody
      • CRISPR-Cas9/Csn1 antibody
      • csn1 antibody
      • SpyCas9 antibody
      see all

    Images

    • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)
      Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)
      All lanes : Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752) at 1/5000 dilution

      Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal) with GFP-tag
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
      Lane 4 : Rat embryo lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 164 kDa


      Exposure time: 3 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected with Streptococcus Thermophilus CRISPR-Cas9 (pcDNA3.1(+)-Myc-His) with ab210752 at 1/50 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed. 

      Positive staining on the 293T cells transfected with Streptococcus Thermophilus Cas9.

       

    • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)
      Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)
      All lanes : Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752) at 1/5000 dilution

      Lane 1 : Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control)
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-tag
      Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-tag
      Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-tag

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 164 kDa


      Exposure time: 5 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The immunogen is located at the N-terminus.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected blank pcDNA3.1(+)-Myc-His vector with ab210752 at 1/50 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed.

      Negative on the 293T cells transfected blank pcDNA3.1(+)-Myc-His vector.

    • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)
      Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)
      All lanes : Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752) at 1/5000 dilution

      Lane 1 : Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control)
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491/LMD-9)) with His-tag
      Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A) with His-tag

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 164 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1-2: 5 seconds; Lane 3: 3 minutes.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab210752? Please let us know so that we can cite the reference in this datasheet.

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