Recombinant
RabMAb

Recombinant Anti-CRISPR-Cas9 antibody [EPR19633] (HRP) (ab215347)

Overview

  • Product name

    Anti-CRISPR-Cas9 antibody [EPR19633] (HRP)
    See all CRISPR-Cas9 primary antibodies
  • Description

    Rabbit monoclonal [EPR19633] to CRISPR-Cas9 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity


    Predicted to work with: Other species
  • Immunogen

    Recombinant fragment within Human CRISPR-Cas9 aa 1000-1100. The exact sequence is proprietary. (Streptococcus thermophilus).
    Database link: G3ECR1

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19633
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab215347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 164, 86 kDa (predicted molecular weight: 164 kDa).

Target

  • Relevance

    [FUNCTION] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
  • Alternative names

    • Cas9 antibody
    • CRISPR-associated endonuclease Cas9/Csn1 antibody
    • CRISPR-Cas9/Csn1 antibody
    • csn1 antibody
    • SpyCas9 antibody
    see all

Images

  • All lanes : Anti-CRISPR-Cas9 antibody [EPR19633] (HRP) (ab215347) at 1/1000 dilution

    Lane 1 : Empty vector with GFP-Myc tag (vector control) transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag
    Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-Myc tag
    Lane 4 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-Myc tag
    Lane 5 : Empty vector with Myc-His tag (vector control) transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 6 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)) with Myc-His tag
    Lane 7 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag
    Lane 8 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)) with Myc-His tag
    Lane 9 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 164 kDa
    Observed band size: 164,86 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST.

References

ab215347 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215347.
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