Key features and details
- Rabbit polyclonal to Crk p38
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Crk p38 antibody
See all Crk p38 primary antibodies
DescriptionRabbit polyclonal to Crk p38
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig
Recombinant fragment corresponding to a region within amino acids 72 and 304 of Crk p38 (UniProt ID: P46108).
- 293T, A431, H1299, HepG2, Raji and NIH 3T3 cell lysates; A431 cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 33 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionThe Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
Sequence similaritiesBelongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
DomainThe C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
The SH2 domain mediates interaction with SHB.
modificationsPhosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
Cellular localizationCytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
- Information by UniProt
- Adapter molecule crk antibody
- Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
- CRK antibody
Anti-Crk p38 antibody (ab151466) at 1/1500 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE
Anti-Crk p38 antibody (ab151466) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE
Immunofluorescence analysis of methanol-fixed A431 cell, labeling Crk p38 using ab151466 at 1/200 dilution.
Lower panel costained with Hoechst 33342.
ab151466 has not yet been referenced specifically in any publications.