Anti-Crk p38 antibody [EP242Y] (ab45136)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Crk p38 antibody [EP242Y]
See all Crk p38 primary antibodies -
Description
Rabbit monoclonal [EP242Y] to Crk p38 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Crk p38 aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- HepG2 cell lysate (WB) and HeLa cells (ICC/IF).
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP242Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab45136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ICC/IF |
Application notes
ICC/IF: 1/250 - 1/500.
IP: 1/50.
WB: 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
Is unsuitable for Flow Cyt or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: 1/50.
WB: 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
Is unsuitable for Flow Cyt or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. -
Sequence similarities
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
The SH2 domain mediates interaction with SHB. -
Post-translational
modificationsPhosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. -
Cellular localization
Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion. - Information by UniProt
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Database links
- Entrez Gene: 1398 Human
- Entrez Gene: 12928 Mouse
- Entrez Gene: 54245 Rat
- Omim: 164762 Human
- SwissProt: P46108 Human
- SwissProt: Q64010 Mouse
- SwissProt: Q63768 Rat
- Unigene: 638121 Human
see all -
Alternative names
- Adapter molecule crk antibody
- Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
- CRK antibody
see all
Images
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![Western blot - Anti-Crk p38 antibody [EP242Y] (ab45136)](https://www.abcam.com/ps/products/45/ab45136/Images/ab45136-57285-anti-crk-p38-antibody-ep242y-western-blot.jpg)
Western blot - Anti-Crk p38 antibody [EP242Y] (ab45136)Anti-Crk p38 antibody [EP242Y] (ab45136) at 1/10000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Crk p38 antibody [EP242Y] (ab45136)](https://www.abcam.com/ps/products/45/ab45136/Images/ab45136-57284-anti-crk-p38-antibody-ep242y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Crk p38 antibody [EP242Y] (ab45136)Immunofluorescent staining of HeLa cells using ab45136 at a 1/250 dilution.
Datasheets and documents
References
This product has been referenced in:
- Ahn J et al. The metastasis gene NEDD9 product acts through integrin ß3 and Src to promote mesenchymal motility and inhibit amoeboid motility. J Cell Sci 125:1814-26 (2012). WB . Read more (PubMed: 22328516) »
