Product nameAnti-Crk p38 antibody [EPR4984(2)]
See all Crk p38 primary antibodies
DescriptionRabbit monoclonal [EPR4984(2)] to Crk p38
The immunogen used for this product shares 79% identity (78% positives) with CRKL. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: WB, Flow Cyt, IP, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a region within Human Crk p38 (UniProt ID: P46108)
- WB: HepG2, K562, HeLa, and Jurkat cell lysates Flow cyt: HepG2 cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
FunctionThe Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
Sequence similaritiesBelongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
DomainThe C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
The SH2 domain mediates interaction with SHB.
modificationsPhosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
Cellular localizationCytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
- Information by UniProt
- Adapter molecule crk antibody
- Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
- CRK antibody
ab133581, at a 1/100, staining Crk p38 in permeabilized HepG2 cells using flow cytometry analysis (Red). Green curve corresponds to a control Rabbit IgG antibody (negative control).
All lanes : Anti-Crk p38 antibody [EPR4984(2)] (ab133581) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab133581 has been referenced in 1 publication.
- Wang Y et al. PTP1B inhibitor promotes endothelial cell motility by activating the DOCK180/Rac1 pathway. Sci Rep 6:24111 (2016). PubMed: 27052191