Overview

  • Product name

  • Description

    Goat polyclonal to CrkL
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Monkey, a wide range of other species, Gorilla, Common marmoset, Bat
  • Immunogen

    Synthetic peptide:

    C-KIFDPQNPDENE

    (with a Cysteine residue linker), corresponding to C terminal amino acids 292-303 of Human CrkL (NP_005198.1; P46109).

  • Positive control

    • Human Thymus tissue, K562 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab129318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 34 kDa.
IHC-P Use a concentration of 2.5 - 3.7 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab129318 at 2.5 µg/ml staining CrkL in Human Thymus tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • Anti-CrkL antibody (ab129318) at 0.01 µg/ml + K562 lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 34 kDa

References

ab129318 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab129318.
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