Product nameAnti-CrkL antibody
See all CrkL primary antibodies
DescriptionGoat polyclonal to CrkL
Tested applicationsSuitable for: WB, ICC, ELISA, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Recombinant Human CrkL protein (ab116208) can be used as a positive control in WB.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab1302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 33 kDa.|
|ICC||Use a concentration of 2.5 - 3.8 µg/ml.|
|ELISA||Use at an assay dependent dilution.|
|IP||Use at an assay dependent concentration.|
FunctionMay mediate the transduction of intracellular signals.
Sequence similaritiesBelongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
Anti-CrkL antibody (ab1302) at 0.01 µg/ml + K562 lysate (35µg protein in RIPA buffer).
Predicted band size: 33 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab1302 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab1302 has not yet been referenced specifically in any publications.