Overview

  • Product name

  • Description

    Rabbit polyclonal to CrkL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-282 of Human CrkL.

  • Positive control

    • 293T, A431, H1299, HeLa S3, HepG2, Molt-4, Raji and Mouse brain whole cell lysates; HeLa cells; Human SW480 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab151791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-CrkL antibody (ab151791) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLaS3 whole cell lysate
    Lane 5 : HepG2 whole cell lysate
    Lane 6 : Molt-4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 34 kDa



    12% SDS PAGE
  • Anti-CrkL antibody (ab151791) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 34 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling CrkL with ab151791 at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling CrkL with ab151791 at 1/500 dilution.

References

This product has been referenced in:

  • Cai YR  et al. Expression level of CRKL and AXL combined with exon 19 deletion in EGFR and ALK status confer differential prognosis of lung adenocarcinoma subtypes. Oncol Lett 12:3312-3322 (2016). IHC-P ; Human . Read more (PubMed: 27899998) »
  • Yu DP  et al. Differential expression of CRKL and AXL genes in lung adenocarcinoma subtypes according to the epidermal growth factor receptor and anaplastic lymphoma kinase gene status. Biomed Rep 2:481-489 (2014). IHC-P ; Human . Read more (PubMed: 24944792) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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