Anti-CrkL antibody (ab151791)
Key features and details
- Rabbit polyclonal to CrkL
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CrkL antibody
See all CrkL primary antibodies -
Description
Rabbit polyclonal to CrkL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-282 of Human CrkL.
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Positive control
- 293T, A431, H1299, HeLa S3, HepG2, Molt-4, Raji and Mouse brain whole cell lysates; HeLa cells; Human SW480 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May mediate the transduction of intracellular signals. -
Sequence similarities
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. - Information by UniProt
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Database links
- Entrez Gene: 1399 Human
- Entrez Gene: 12929 Mouse
- Entrez Gene: 287942 Rat
- Omim: 602007 Human
- SwissProt: P46109 Human
- SwissProt: P47941 Mouse
- SwissProt: Q5U2U2 Rat
- Unigene: 5613 Human
see all -
Alternative names
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
see all
Images
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All lanes : Anti-CrkL antibody (ab151791) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLaS3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : Molt-4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
12% SDS PAGE -
Anti-CrkL antibody (ab151791) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 34 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling CrkL with ab151791 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling CrkL with ab151791 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab151791 has been referenced in 4 publications.
- Jiang Y et al. ZEB2-AS1 Accelerates Epithelial/Mesenchymal Transition Through miR-1205/CRKL Pathway in Colorectal Cancer. Cancer Biother Radiopharm 35:153-162 (2020). PubMed: 31755734
- Civciristov S et al. Ligand-dependent spatiotemporal signaling profiles of the µ-opioid receptor are controlled by distinct protein-interaction networks. J Biol Chem 294:16198-16213 (2019). PubMed: 31515267
- Cai YR et al. Expression level of CRKL and AXL combined with exon 19 deletion in EGFR and ALK status confer differential prognosis of lung adenocarcinoma subtypes. Oncol Lett 12:3312-3322 (2016). IHC-P ; Human . PubMed: 27899998
- Yu DP et al. Differential expression of CRKL and AXL genes in lung adenocarcinoma subtypes according to the epidermal growth factor receptor and anaplastic lymphoma kinase gene status. Biomed Rep 2:481-489 (2014). IHC-P ; Human . PubMed: 24944792