Overview

  • Product name

    Anti-CrkL antibody [Y243]
    See all CrkL primary antibodies
  • Description

    Rabbit monoclonal [Y243] to CrkL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the SH2 domain, near the N-term of human CrkL

  • Positive control

    • K562 cell lysate and human breast carcinoma
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      May mediate the transduction of intracellular signals.
    • Sequence similarities

      Belongs to the CRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Information by UniProt
    • Database links

    • Alternative names

      • Crk L antibody
      • Crk like protein antibody
      • Crk-like protein antibody
      • Crkl antibody
      • CRKL_HUMAN antibody
      • Crkol antibody
      • HGNC:2363 antibody
      • Oncogene CrkL antibody
      • V crk avian sarcoma virus CT10 oncogene homolog like antibody
      • v crk sarcoma virus CT10 oncogene homolog (avian) like antibody
      • V crk sarcoma virus CT10 oncogene homolog avian like antibody
      see all

    Images

    • Anti-CrkL antibody [Y243] (ab32126) at 1/1000 dilution + K562 cell lysate

      Predicted band size: 33 kDa
      Observed band size: 39 kDa
      why is the actual band size different from the predicted?

    • ab32126, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Wang L  et al. Expression of signaling adaptor proteins predicts poor prognosis in pancreatic ductal adenocarcinoma. Diagn Pathol 12:42 (2017). Read more (PubMed: 28558797) »
    • Poulsen ET  et al. New Insights to Clathrin and Adaptor Protein 2 for the Design and Development of Therapeutic Strategies. Int J Mol Sci 16:29446-53 (2015). IP . Read more (PubMed: 26690411) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for your inquiry. I have confirmed that neither of these two anti-CrkL antibodies, ab32018 or ab32126, bind within the region a.a. 123 - 183 (the first SH3 domain). I apologize but it doesn't look like any of the anti-CrkL antibodies that we currently carry target the first SH3 domain. You may want to try searching www.biocompare.com to find one that does. Please feel free to contact me if you have other questions.

    Read More

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