Product nameAnti-CrkL antibody [Y244]
See all CrkL primary antibodies
DescriptionRabbit monoclonal [Y244] to CrkL
Specificityab32018 recognises CrkL (Crk-like)protein.
Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CrkL aa 250-350 (C terminal). The exact sequence is proprietary.
- K562 cell lysate, human metastatic lung carcinoma
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay mediate the transduction of intracellular signals.
Sequence similaritiesBelongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
ab32018, at a 1/50 dilution, staining human Crk in metastatic lung carcinoma by Immunohistochemistry, in paraffin embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CrkL antibody [Y244] (ab32018) at 1/500 dilution + K562 cell lysate
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Hutchinson CV et al. Lymphocytes from chronic lymphocytic leukaemia undergo ABL1-linked amoeboid motility and homotypic interaction as an early adaptive change to ex vivo culture. Exp Hematol Oncol 3:7 (2014). Read more (PubMed: 24618035) »