Overview

  • Product name

    Anti-CrkL antibody [Y244]
    See all CrkL primary antibodies
  • Description

    Rabbit monoclonal [Y244] to CrkL
  • Host species

    Rabbit
  • Specificity

    ab32018 recognises CrkL (Crk-like)protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CrkL aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • K562 cell lysate, human metastatic lung carcinoma
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50.
IP 1/10.

Target

  • Function

    May mediate the transduction of intracellular signals.
  • Sequence similarities

    Belongs to the CRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • Crk L antibody
    • Crk like protein antibody
    • Crk-like protein antibody
    • Crkl antibody
    • CRKL_HUMAN antibody
    • Crkol antibody
    • HGNC:2363 antibody
    • Oncogene CrkL antibody
    • V crk avian sarcoma virus CT10 oncogene homolog like antibody
    • v crk sarcoma virus CT10 oncogene homolog (avian) like antibody
    • V crk sarcoma virus CT10 oncogene homolog avian like antibody
    see all

Images

  • ab32018, at a 1/50 dilution, staining human Crk in metastatic lung carcinoma by Immunohistochemistry, in paraffin embedded tissue

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-CrkL antibody [Y244] (ab32018) at 1/500 dilution + K562 cell lysate

    Predicted band size: 33 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Hutchinson CV  et al. Lymphocytes from chronic lymphocytic leukaemia undergo ABL1-linked amoeboid motility and homotypic interaction as an early adaptive change to ex vivo culture. Exp Hematol Oncol 3:7 (2014). Read more (PubMed: 24618035) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry. I have confirmed that neither of these two anti-CrkL antibodies, ab32018 or ab32126, bind within the region a.a. 123 - 183 (the first SH3 domain). I apologize but it doesn't look like any of the anti-CrkL antibodies that we currently carry target the first SH3 domain. You may want to try searching www.biocompare.com to find one that does. Please feel free to contact me if you have other questions.

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