Product nameAnti-CrkL (phospho Y207) antibody [EP270Y]
See all CrkL primary antibodies
DescriptionRabbit monoclonal [EP270Y] to CrkL (phospho Y207)
Specificityab52908 only detects CrkL phosphorylated on Tyrosine 207.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Human
A synthetic phospho peptide corresponding to residues surrounding Tyr207 of human CrkL
- WB: K562 cell lysates
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab52908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa).|
FunctionMay mediate the transduction of intracellular signals.
Sequence similaritiesBelongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
All lanes : Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908) at 1/50000 dilution
Lane 1 : (A) K562 cell lysates untreated
Lane 2 : (B) K562 cell lysates treated with AP
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Ouellette SB et al. A Cell-Based Assay for Measuring Endogenous BcrAbl Kinase Activity and Inhibitor Resistance. PLoS One 11:e0161748 (2016). Read more (PubMed: 27598410) »
- Li J et al. Inhibition of 32Dp210 cells harboring T315I mutation by a novel derivative of emodin correlates with down-regulation of BCR-ABL and its downstream signaling pathways. J Cancer Res Clin Oncol N/A:N/A (2014). Human . Read more (PubMed: 25217883) »