Anti-CrkL (phospho Y251) antibody (ab215751)

Overview

  • Product name
    Anti-CrkL (phospho Y251) antibody
    See all CrkL primary antibodies
  • Description
    Rabbit polyclonal to CrkL (phospho Y251)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Synthetic peptide within Human CrkL (phospho Y251) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P46109

  • Positive control
    • K562 cell lysate stimulated with pervanadate.

Properties

Applications

Our Abpromise guarantee covers the use of ab215751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 34 kDa.

Target

Images

  • Lanes 1-2 : Rabbit anti-CrkL (C-terminus)
    Lanes 3-4 : Anti-CrkL (phospho Y251) antibody (ab215751) at 1/1000 dilution

    Lanes 1 & 3 : K562 cell lysate stimulated with pervanadate (1mM) for 30 min
    Lanes 2 & 4 : K562 cell lysate stimulated with pervanadate (1mM) for 30 min treated with alkaline phosphatase

    Predicted band size: 34 kDa
    Observed band size: 38, 40 kDa (why is the actual band size different from the predicted?)

References

ab215751 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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