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Rabbit polyclonal to CrkL (phospho Y251)
HumanPredicted to work with:
Mouse, Rat, Chicken
Synthetic peptide within Human CrkL (phospho Y251) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P46109
- K562 cell lysate stimulated with pervanadate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000. Predicted molecular weight: 34 kDa.
May mediate the transduction of intracellular signals.
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Information by UniProt
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
Western blot - Anti-CrkL (phospho Y251) antibody (ab215751)
Lanes 1-2 :
Rabbit anti-CrkL (C-terminus)Lanes 3-4 :
Anti-CrkL (phospho Y251) antibody (ab215751) at 1/1000 dilutionLanes 1 & 3 :
K562 cell lysate stimulated with pervanadate (1mM) for 30 minLanes 2 & 4 :
K562 cell lysate stimulated with pervanadate (1mM) for 30 min treated with alkaline phosphatasePredicted band size:
34 kDaObserved band size:
38, 40 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"