Product nameAnti-CrkL (phospho Y251) antibody
See all CrkL primary antibodies
DescriptionRabbit polyclonal to CrkL (phospho Y251)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken
Synthetic peptide within Human CrkL (phospho Y251) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P46109
- K562 cell lysate stimulated with pervanadate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab215751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 34 kDa.|
FunctionMay mediate the transduction of intracellular signals.
Sequence similaritiesBelongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
Lanes 1-2 : Rabbit anti-CrkL (C-terminus)
Lanes 3-4 : Anti-CrkL (phospho Y251) antibody (ab215751) at 1/1000 dilution
Lanes 1 & 3 : K562 cell lysate stimulated with pervanadate (1mM) for 30 min
Lanes 2 & 4 : K562 cell lysate stimulated with pervanadate (1mM) for 30 min treated with alkaline phosphatase
Predicted band size: 34 kDa
Observed band size: 38, 40 kDa why is the actual band size different from the predicted?
ab215751 has not yet been referenced specifically in any publications.