Key features and details
- Rabbit polyclonal to CrkL (phospho Y251)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CrkL (phospho Y251) antibody
See all CrkL primary antibodies
DescriptionRabbit polyclonal to CrkL (phospho Y251)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken
Synthetic peptide within Human CrkL (phospho Y251) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P46109
- K562 cell lysate stimulated with pervanadate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab215751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 34 kDa.|
FunctionMay mediate the transduction of intracellular signals.
Sequence similaritiesBelongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
Lanes 1-2 : Rabbit anti-CrkL (C-terminus)
Lanes 3-4 : Anti-CrkL (phospho Y251) antibody (ab215751) at 1/1000 dilution
Lanes 1 & 3 : K562 cell lysate stimulated with pervanadate (1mM) for 30 min
Lanes 2 & 4 : K562 cell lysate stimulated with pervanadate (1mM) for 30 min treated with alkaline phosphatase
Predicted band size: 34 kDa
Observed band size: 38, 40 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab215751 has not yet been referenced specifically in any publications.