Anti-CRLF2 antibody (ab109626)
Key features and details
- Rabbit polyclonal to CRLF2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CRLF2 antibody
See all CRLF2 primary antibodies -
Description
Rabbit polyclonal to CRLF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
KLH conjugated synthetic peptide of 19 amino acids in length from near the center of Human CRLF2.
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Positive control
- Human liver tissue, Human kidney tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system. -
Tissue specificity
Expressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is important for association with JAKs. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 64109 Human
- Omim: 300357 Human
- Omim: 400023 Human
- SwissProt: Q9HC73 Human
- Unigene: 287729 Human
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Alternative names
- CRL 2 antibody
- CRL2 antibody
- CRLF 2 antibody
see all
Images
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Human kidney tissue (formalin-fixed, paraffin-embedded) stained with ab109626 at a concentration of 5 µg/ml.
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Lane 1 : Anti-CRLF2 antibody (ab109626) at 0.5 µg/ml
Lane 2 : Anti-CRLF2 antibody (ab109626) at 1 µg/ml
Lane 3 : Anti-CRLF2 antibody (ab109626) at 2 µg/ml
All lanes : Human liver tissue lysate
Predicted band size: 42 kDa -
Human liver tissue (formalin-fixed, paraffin-embedded) stained with ab109626 at a concentration of 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab109626 has been referenced in 2 publications.
- Jiang M et al. Potential efficacy and prognosis of silencing the CRLF2-mediated AKT/mTOR pathway in pediatric acute B-cell lymphoblastic leukemia. Oncol Rep 41:885-894 (2019). PubMed: 30535452
- Yamauchi M et al. Myeloid dendritic cells stimulated by thymic stromal lymphopoietin promote Th2 immune responses and the pathogenesis of oral lichen planus. PLoS One 12:e0173017 (2017). IHC . PubMed: 28278185