Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14603] to CRLF2 - C-terminal
- Suitable for: WB
- Reacts with: Human
Product nameAnti-CRLF2 antibody [EPR14603] - C-terminal
See all CRLF2 primary antibodies
DescriptionRabbit monoclonal [EPR14603] to CRLF2 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CRLF2 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: Q9HC73
- Human fetal kidney, THP1, HepG2 and Jurkat cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab186427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 42 kDa).|
FunctionReceptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.
Tissue specificityExpressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 5 subfamily.
Contains 1 fibronectin type-III domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is important for association with JAKs.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CRL 2 antibody
- CRL2 antibody
- CRLF 2 antibody
All lanes : Anti-CRLF2 antibody [EPR14603] - C-terminal (ab186427) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : THP1 lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Blocking buffer and concentration: 5% NFDM/TBST
ab186427 has not yet been referenced specifically in any publications.