Recombinant
RabMAb

Recombinant Anti-CRLF2 antibody [EPR14603] - C-terminal (ab186427)

Overview

  • Product name

    Anti-CRLF2 antibody [EPR14603] - C-terminal
    See all CRLF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14603] to CRLF2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CRLF2 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9HC73

  • Positive control

    • Human fetal kidney, THP1, HepG2 and Jurkat cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14603
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 42 kDa).

Target

  • Function

    Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.
  • Tissue specificity

    Expressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 5 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is important for association with JAKs.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CRL 2 antibody
    • CRL2 antibody
    • CRLF 2 antibody
    • Crlf2 antibody
    • CRLF2_HUMAN antibody
    • CRLF2Y antibody
    • Cytokine receptor CRL2 precusor antibody
    • Cytokine receptor like 2 antibody
    • Cytokine receptor like factor 2 antibody
    • Cytokine receptor like factor 2 precursor antibody
    • Cytokine receptor-like 2 antibody
    • Cytokine receptor-like factor 2 antibody
    • IL XR antibody
    • IL-XR antibody
    • ILXR antibody
    • Thymic stromal derived lymphopoietin receptor antibody
    • Thymic stromal lymphopoietin protein receptor antibody
    • Thymic stromal lymphopoietin receptor antibody
    • TSLP receptor antibody
    • TSLPR antibody
    see all

Images

  • All lanes : Anti-CRLF2 antibody [EPR14603] - C-terminal (ab186427) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : THP1 lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST

References

ab186427 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab186427.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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