Recombinant
RabMAb

Recombinant Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)

Overview

  • Product name

    Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free
    See all CRLF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR22478-133] to CRLF2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CRLF2 aa 50-150. The exact sequence is proprietary.
    Database link: Q9HC73

  • Positive control

    • Flow Cyt: Human PBMCs.
  • General notes

    Ab256305 is the carrier-free version of ab224196. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab256305 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22478-133
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab256305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P,IP or WB.
  • Target

    • Function

      Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.
    • Tissue specificity

      Expressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells.
    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 5 subfamily.
      Contains 1 fibronectin type-III domain.
    • Domain

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is important for association with JAKs.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CRL 2 antibody
      • CRL2 antibody
      • CRLF 2 antibody
      • Crlf2 antibody
      • CRLF2_HUMAN antibody
      • CRLF2Y antibody
      • Cytokine receptor CRL2 precusor antibody
      • Cytokine receptor like 2 antibody
      • Cytokine receptor like factor 2 antibody
      • Cytokine receptor like factor 2 precursor antibody
      • Cytokine receptor-like 2 antibody
      • Cytokine receptor-like factor 2 antibody
      • IL XR antibody
      • IL-XR antibody
      • ILXR antibody
      • Thymic stromal derived lymphopoietin receptor antibody
      • Thymic stromal lymphopoietin protein receptor antibody
      • Thymic stromal lymphopoietin receptor antibody
      • TSLP receptor antibody
      • TSLPR antibody
      see all

    Images

    • Flow cytometric analysis of human peripheral blood mononuclear cells labeling CRLF2 with ab224196 at 1/60 dilution (Right compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

      Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab224196 (Right). Then stained with anti-CD11c conjugated to Alexa Fluor® 647.

      Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224196).

    • Flow cytometric analysis of human peripheral blood mononuclear cells labeling CRLF2 with ab224196 at 1/60 dilution (Right compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

      Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab224196 (Right). Then stained with anti-CD14 conjugated to Alexa Fluor® 647.

      Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224196).

    • Flow cytometric analysis of LNCaP (human prostate cancer cell line) cells labeling CRLF2 with ab224196 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

      Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

      Negative control: LNCaP (PMID: 20236697). Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224196).

    References

    ab256305 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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