Overview

  • Product name

  • Description

    Rabbit polyclonal to CRLR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CRLR aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab99160)

  • Positive control

    • This antibody gave a positive signal in human liver tissue and A549 whole cell lysates. IHC-P: human lung tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab84467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 53 kDa).

Target

  • Relevance

    CRLR is a receptor for calcitonin gene related peptide type 1. The activity of CRLR is mediated by G proteins which activate adenylyl cyclase. CRLR expression has been reported in brain, lung, blood vessel, liver, and intestinal tract. ESTs have been isolated from B Cell/lung/testis, bone marrow, embryo, lung, and synovium libraries.
  • Cellular localization

    Cell Membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • Calcitonin gene related peptide type 1 receptor antibody
    • Calcitonin receptor like antibody
    • Calcitonin receptor like receptor antibody
    • CALCRL antibody
    • CGRP type 1 receptor antibody
    • CGRPR antibody
    • CRLR antibody
    see all

Images

  • All lanes : Anti-CRLR antibody (ab84467) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    Human Calcitonin gene-related peptide type 1 receptor precursor (CRLR)contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • IHC image of CRLR staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84467, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Yan D  et al. Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis. Sci Rep 9:2698 (2019). Read more (PubMed: 30804432) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thanks for your call today and for letting us know about the trouble with ab84467. As we discussed, I would recommend using a higher concentration of secondary antibody, and checking that the other components of the assay have been working with other primary antibodies. If there are still no bands on the blot, I would be happy to send a replacement or issue a credit or refund. I look forward to hearing from you, and please let me know if you have any questions.

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