Western blot abreview for Anti-CRM1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk/TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL, Pico
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 117 kDa

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Submitted Sep 01 2010

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