- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-CRM1 antibody
See all CRM1 primary antibodies -
DescriptionRabbit polyclonal to CRM1
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WB, ICCmore details
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Species reactivityReacts with: Cow, Human
Predicted to work with: Mouse, Rat, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human CRM1 aa 1043-1058.
Sequence:RQADEEKHKRQMSVPGS
(Peptide available asab41768)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Primary antibody notesDiffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC’s. NPC’s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab3459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.4 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 119 kDa).Can be blocked with Human CRM1 peptide (ab41768). | |
| ICC | 1/1000. |
Target
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FunctionMediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.
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Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.
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Sequence similaritiesBelongs to the exportin family.
Contains 10 HEAT repeats.
Contains 1 importin N-terminal domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 510568 Cow
- Entrez Gene: 7514 Human
- Entrez Gene: 103573 Mouse
- Entrez Gene: 85252 Rat
- Entrez Gene: 399087 Xenopus laevis
- Omim: 602559 Human
- SwissProt: O14980 Human
- SwissProt: Q6P5F9 Mouse
see all -
Alternative names
- Chromosome region maintenance 1 protein homolog antibody
- CRM 1 antibody
- CRM1 homolog antibody
see all
Images
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Western blot - Anti-CRM1 antibody (ab3459)All lanes : Anti-CRM1 antibody (ab3459) at 1/1000 dilution
Lane 1 : SK-BR-3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH-3T3 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit
Predicted band size: 119 kDa
Observed band size: 130 kDa (why is the actual band size different from the predicted?)
Protocols
References
This product has been referenced in:
- Lussi YC et al. The nucleoporin Nup88 is interacting with nuclear lamin A. Mol Biol Cell 22:1080-90 (2011). Read more (PubMed: 21289091) »
- Castanotto D et al. CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs. Proc Natl Acad Sci U S A 106:21655-9 (2009). IP ; Human . Read more (PubMed: 19955415) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"