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    crm1-antibody-ab3459.pdf

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Signal Transduction Protein Trafficking Nuclear Import / Export
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Anti-CRM1 antibody (ab3459)

  • Datasheet
Reviews (1) Submit a question References (4)

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Abpromise

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Western blot - Anti-CRM1 antibody (ab3459)

    Key features and details

    • Rabbit polyclonal to CRM1
    • Suitable for: WB
    • Reacts with: Mouse, Cow
    • Isotype: IgG

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    Overview

    • Product name

      Anti-CRM1 antibody
      See all CRM1 primary antibodies
    • Description

      Rabbit polyclonal to CRM1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Cow
    • Immunogen

      Synthetic peptide corresponding to Human CRM1 aa 1043-1058.
      Sequence:

      RQADEEKHKRQMSVPGS


      (Peptide available as ab41768)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Primary antibody notes

      Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC’s. NPC’s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Nuclear Import / Export
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • Other
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Nuclear Pore Complex

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human CRM1 protein (ab131897)

    Applications

    Our Abpromise guarantee covers the use of ab3459 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 119 kDa).

    Target

    • Function

      Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.
    • Tissue specificity

      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.
    • Sequence similarities

      Belongs to the exportin family.
      Contains 10 HEAT repeats.
      Contains 1 importin N-terminal domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
    • Target information above from: UniProt accession O14980 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 510568 Cow
      • Entrez Gene: 103573 Mouse
      • SwissProt: Q6P5F9 Mouse
      • Unigene: 217547 Mouse
      • Alternative names

        • Chromosome region maintenance 1 protein homolog antibody
        • CRM 1 antibody
        • CRM1 homolog antibody
        • DKFZp686B1823 antibody
        • emb antibody
        • Exp 1 antibody
        • Exp1 antibody
        • Exportin 1 antibody
        • Exportin-1 antibody
        • Exportin1 antibody
        • XPO 1 antibody
        • xpo1 antibody
        • XPO1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-CRM1 antibody (ab3459)
        Western blot - Anti-CRM1 antibody (ab3459)
        All lanes : Anti-CRM1 antibody (ab3459) at 1/1000 dilution

        Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate
        Lane 2 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
        Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

        Lysates/proteins at 25 µg per lane.

        Secondary
        All lanes : HRP-conjugated anti-rabbit

        Predicted band size: 119 kDa
        Observed band size: 130 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (4)

      Publishing research using ab3459? Please let us know so that we can cite the reference in this datasheet.

      ab3459 has been referenced in 4 publications.

      • Lussi YC  et al. The nucleoporin Nup88 is interacting with nuclear lamin A. Mol Biol Cell 22:1080-90 (2011). PubMed: 21289091
      • Castanotto D  et al. CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs. Proc Natl Acad Sci U S A 106:21655-9 (2009). IP ; Human . PubMed: 19955415
      • Lai MC  et al. The DEAD-box RNA helicase DDX3 associates with export messenger ribonucleoproteins as well as tip-associated protein and participates in translational control. Mol Biol Cell 19:3847-58 (2008). ICC/IF ; Human . PubMed: 18596238
      • Mostaqul Huq MD  et al. Suppression of receptor interacting protein 140 repressive activity by protein arginine methylation. EMBO J 25:5094-104 (2006). WB . PubMed: 17053781

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunocytochemistry abreview for Anti-CRM1 antibody

      Excellent
      Abreviews
      Abreviews
      Application
      Immunocytochemistry
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 06 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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