Anti-CRM1 antibody (ab84375)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 antibody (ab84375)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 antibody (ab84375)

Key features and details

  • Rabbit polyclonal to CRM1
  • Suitable for: IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CRM1 antibody
    See all CRM1 primary antibodies

  • Description

    Rabbit polyclonal to CRM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 1025-1071 (FAGEDTSDLF LEEREIALRQ ADEEKHKRQM SVPGIFNPHE IPEEMCD) of human CRM1 (NP_003391.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human breast tumor; mouse squamous cell carcinoma.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab84375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Notes
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.

    • Tissue specificity

      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.

    • Sequence similarities

      Belongs to the exportin family.
      Contains 10 HEAT repeats.
      Contains 1 importin N-terminal domain.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Chromosome region maintenance 1 protein homolog antibody
      • CRM 1 antibody
      • CRM1 homolog antibody
      • DKFZp686B1823 antibody
      • emb antibody
      • Exp 1 antibody
      • Exp1 antibody
      • Exportin 1 antibody
      • Exportin-1 antibody
      • Exportin1 antibody
      • XPO 1 antibody
      • xpo1 antibody
      • XPO1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 antibody (ab84375)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 antibody (ab84375)
      ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded human breast tumor section by immunohistochemistry.
      Detection: DAB staining
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 antibody (ab84375)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 antibody (ab84375)
      ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded mouse squamous cell carcinoma by immunohistochemistry. Detection: DAB staining

    References (1)

    Publishing research using ab84375? Please let us know so that we can cite the reference in this datasheet.

    ab84375 has been referenced in 1 publication.

    • Heerma van Voss MR  et al. Nuclear DDX3 expression predicts poor outcome in colorectal and breast cancer. Onco Targets Ther 10:3501-3513 (2017). PubMed: 28761359

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Could you update me about my questions about CRM1 antibody?

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    Answer

    Thank you for your enquiry. I wish to sincerely apologize for the delay.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, anyanti-CRM1 antibody tested in the species and application you are using should be suitable for your applications.

    Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet). My personal feeling is that the images for ab84375 look better, there are more application notes for IHC and more positive control choices, as such I would likely choose this product.

    The IgG control for Anti-CRM1 ab84375 would be Rabbit polyclonal IgG ab27472. More information about this may be found at the following location:

    https://www.abcam.com/Rabbit-polyclonal-IgG-ab27472.html

    I hope this information is helpful. Should you have any further questions please do not hesitate to ask.

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    Question

    I am looking for a CRM1 antibody for IHC-P and found two of them on your website (ab77977 and ab84375). Which antibody would recommend for IHC-P? As much as I see the sample pictures, the staining with ab84375 looks more clear than the one with ab77977.   Could you also give me a catalog number for a control IgG which is appropriate these CRM1 antibodies?

    Read More
    Answer

    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any CRM1 antibody tested in the species and application you are using should be suitable for your applications.

    Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet). However in this case I do agree that ab84375 does look clearer.

    I can recommened our Rabbit polyclonal IgG- ChIP Grade, ab27478 as a control IgG for either product. More information about this may be found at this location:

    https://www.abcam.com/rabbit-polyclonal-igg-ab27478.html

    Please know that these products are covered by our Abpromise guarantee. We are happy to provide scientific support, replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

    https://www.abcam.com/index.html?pageconfig=abpromise



    I look forward to hearing from you. Should you have any further questions please do not hesitate to ask.

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