Anti-CRM1 antibody (ab84375)
Key features and details
- Rabbit polyclonal to CRM1
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CRM1 antibody
See all CRM1 primary antibodies -
Description
Rabbit polyclonal to CRM1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to a region within C terminal amino acids 1025-1071 (FAGEDTSDLF LEEREIALRQ ADEEKHKRQM SVPGIFNPHE IPEEMCD) of human CRM1 (NP_003391.1).
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Positive control
- Human breast tumor; mouse squamous cell carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified using an epitope specific to CRM1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Notes |
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Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney. -
Sequence similarities
Belongs to the exportin family.
Contains 10 HEAT repeats.
Contains 1 importin N-terminal domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 459534 Chimpanzee
- Entrez Gene: 7514 Human
- Entrez Gene: 103573 Mouse
- Entrez Gene: 718063 Rhesus monkey
- Omim: 602559 Human
- SwissProt: O14980 Human
- SwissProt: Q6P5F9 Mouse
- Unigene: 370770 Human
see all -
Alternative names
- Chromosome region maintenance 1 protein homolog antibody
- CRM 1 antibody
- CRM1 homolog antibody
see all
Images
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ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded human breast tumor section by immunohistochemistry.
Detection: DAB staining -
ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded mouse squamous cell carcinoma by immunohistochemistry. Detection: DAB staining
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab84375 has been referenced in 1 publication.
- Heerma van Voss MR et al. Nuclear DDX3 expression predicts poor outcome in colorectal and breast cancer. Onco Targets Ther 10:3501-3513 (2017). PubMed: 28761359