Overview

  • Product name

    Anti-CRM1 antibody [EPR14123(B)]
    See all CRM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14123(B)] to CRM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CRM1 aa 50-150. The exact sequence is proprietary.
    Database link: O14980

  • Positive control

    • K562, HeLa, and Jurkat cell lysate. K562 and HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 123 kDa).
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/500.

Target

  • Function

    Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.
  • Sequence similarities

    Belongs to the exportin family.
    Contains 10 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chromosome region maintenance 1 protein homolog antibody
    • CRM 1 antibody
    • CRM1 homolog antibody
    • DKFZp686B1823 antibody
    • emb antibody
    • Exp 1 antibody
    • Exp1 antibody
    • Exportin 1 antibody
    • Exportin-1 antibody
    • Exportin1 antibody
    • XPO 1 antibody
    • xpo1 antibody
    • XPO1_HUMAN antibody
    see all

Images

  • All lanes : Anti-CRM1 antibody [EPR14123(B)] (ab180144) at 1/2000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 123 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5%NFDM/TBST

  • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling CRM1 with Purified ab180144 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling CRM1 with ab180144 at 1/200. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG.

  • Immunofluorescent analysis of 20°C acetone fixed K562 cells labeling CRM1 with ab180144 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 488) .

References

ab180144 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Drosophila melanogaster Cell lysate - whole cell (whole tissue thoraces and S2 cells)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
10 µg
Specification
whole tissue thoraces and S2 cells
Blocking step
Casein sodium salt as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.4% · Temperature: 25°C

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Verified customer

Submitted Jul 12 2019

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