Overview

  • Product name

    Anti-CRM1 antibody [EPR14124] - C-terminal
    See all CRM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14124] to CRM1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CRM1 aa 1000 to the C-terminus. The exact sequence is proprietary.
    Database link: O14980

  • Positive control

    • K562, HeLa, Jurkat, Ramos, C6, NIH 3T3 and PC12 cell lysates; Human bladder transitional cell carcinoma tissue; HeLa and K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50.
WB 1/1000 - 1/20000. Detects a band of approximately 110 kDa (predicted molecular weight: 123 kDa).

Target

  • Function

    Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.
  • Sequence similarities

    Belongs to the exportin family.
    Contains 10 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chromosome region maintenance 1 protein homolog antibody
    • CRM 1 antibody
    • CRM1 homolog antibody
    • DKFZp686B1823 antibody
    • emb antibody
    • Exp 1 antibody
    • Exp1 antibody
    • Exportin 1 antibody
    • Exportin-1 antibody
    • Exportin1 antibody
    • XPO 1 antibody
    • xpo1 antibody
    • XPO1_HUMAN antibody
    see all

Images

  • All lanes : Anti-CRM1 antibody [EPR14124] - C-terminal (ab191081) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 123 kDa

  • Immunohistochemical analysis of paraffin-embedded human bladder transitional cell carcinoma tissue labeling CRM1 with ab191081 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).

  • Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100-permeabilized) labeling CRM1 with ab191081 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow cytometric analysis of K562 cells (paraformaldehyde-fixed, 2%) labeling CRM1 with ab191081 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • All lanes : Anti-CRM1 antibody [EPR14124] - C-terminal (ab191081) at 1/20000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 123 kDa

References

This product has been referenced in:

  • Barazeghi E  et al. Decrease of 5-hydroxymethylcytosine and TET1 with nuclear exclusion of TET2 in small intestinal neuroendocrine tumors. BMC Cancer 18:764 (2018). Read more (PubMed: 30045709) »
  • Xie QL  et al. Chromosome region maintenance 1 expression and its association with clinical pathological features in primary carcinoma of the liver. Exp Ther Med 12:59-68 (2016). Human . Read more (PubMed: 27347018) »
See all 2 Publications for this product

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