Anti-CRMP1 antibody (ab228669)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-CRMP1 antibody
See all CRMP1 primary antibodies -
Description
Rabbit polyclonal to CRMP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Rhesus monkey
-
Immunogen
Recombinant fragment within Human CRMP1 (internal sequence). The exact sequence is proprietary.
Database link: Q14194 -
Positive control
- IHC-P: U87 xenograft tissue; mouse forebrain tissue. WB: Mouse brain whole cell lysate; HepG2 whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab228669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/50000. Predicted molecular weight: 62 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
-
Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes and the mitotic spindle during metaphase. - Information by UniProt
-
Database links
- Entrez Gene: 395156 Chicken
- Entrez Gene: 1400 Human
- Entrez Gene: 12933 Mouse
- Entrez Gene: 25415 Rat
- Entrez Gene: 711349 Rhesus monkey
- Omim: 602462 Human
- SwissProt: Q14194 Human
- SwissProt: P97427 Mouse
see all -
Alternative names
- Collapsin response mediator protein 1 antibody
- CRMP 1 antibody
- CRMP-1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody (ab228669)Paraffin-embedded U87 xenograft tissue stained for CRMP1 with ab228669 at 1/500 dilution in immunohistochemical analysis.
-
Western blot - Anti-CRMP1 antibody (ab228669)Anti-CRMP1 antibody (ab228669) at 1/5000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 62 kDa7.5% SDS-PAGE
-
Western blot - Anti-CRMP1 antibody (ab228669)Anti-CRMP1 antibody (ab228669) at 1/50000 dilution + Mouse brain whole cell lysate at 1 µg
Developed using the ECL technique.
Predicted band size: 62 kDa7.5% SDS-PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody (ab228669)Paraffin-embedded mouse forebrain tissue stained for CRMP1 with ab228669 at a 1/500 dilution in immunohistochemical analysis.
References
ab228669 has not yet been referenced specifically in any publications.
