Anti-CRMP1 antibody (ab228669)
Key features and details
- Rabbit polyclonal to CRMP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CRMP1 antibody
See all CRMP1 primary antibodies -
Description
Rabbit polyclonal to CRMP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Rhesus monkey
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Immunogen
Recombinant fragment within Human CRMP1 (internal sequence). The exact sequence is proprietary.
Database link: Q14194 -
Positive control
- IHC-P: U87 xenograft tissue; mouse forebrain tissue. WB: Mouse brain whole cell lysate; HepG2 whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab228669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/50000. Predicted molecular weight: 62 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes and the mitotic spindle during metaphase. - Information by UniProt
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Database links
- Entrez Gene: 395156 Chicken
- Entrez Gene: 1400 Human
- Entrez Gene: 12933 Mouse
- Entrez Gene: 25415 Rat
- Entrez Gene: 711349 Rhesus monkey
- Omim: 602462 Human
- SwissProt: Q14194 Human
- SwissProt: P97427 Mouse
see all -
Alternative names
- Collapsin response mediator protein 1 antibody
- CRMP 1 antibody
- CRMP-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody (ab228669)Paraffin-embedded U87 xenograft tissue stained for CRMP1 with ab228669 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-CRMP1 antibody (ab228669)Anti-CRMP1 antibody (ab228669) at 1/5000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 62 kDa7.5% SDS-PAGE
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Western blot - Anti-CRMP1 antibody (ab228669)Anti-CRMP1 antibody (ab228669) at 1/50000 dilution + Mouse brain whole cell lysate at 1 µg
Developed using the ECL technique.
Predicted band size: 62 kDa7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody (ab228669)Paraffin-embedded mouse forebrain tissue stained for CRMP1 with ab228669 at a 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab228669 has not yet been referenced specifically in any publications.
