Key features and details
- Mouse monoclonal [1B1] to CRMP2
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-CRMP2 antibody [1B1]
See all CRMP2 primary antibodies
DescriptionMouse monoclonal [1B1] to CRMP2
Does not react with CRMP-1, CRMP-3, CRMP-4 or CRMP-5.
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Immunogen: A 15 residue synthetic peptide derived from C-terminal domain of human collapsing-response mediator protein Type 2 (MW: 65 kDa).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 1% Sucrose, 1.21% Tris, 0.375% Glycine
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab62539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
modifications3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
- Information by UniProt
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
Anti-CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 65 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Overlay histogram showing SH-SY5Y cells stained with ab62539 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62539, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab62539 has been referenced in 2 publications.
- Zhou K et al. NMDA receptor hypofunction induces dysfunctions of energy metabolism and semaphorin signaling in rats: a synaptic proteome study. Schizophr Bull 38:579-91 (2012). WB ; Rat . PubMed: 21084551
- Chi XX et al. Regulation of N-type voltage-gated calcium channels (Cav2.2) and transmitter release by collapsin response mediator protein-2 (CRMP-2) in sensory neurons. J Cell Sci 122:4351-62 (2009). PubMed: 19903690