Overview

  • Product name

  • Description

    Rabbit polyclonal to CRMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide:

    DFVYKRIKARSRLAELRG

    conjugated to KLH, corresponding to amino acids 476-493 of Human CRMP2

  • Positive control

    • Hela whole cell lysate; mouse brain extract

Properties

Applications

Our Abpromise guarantee covers the use of ab62661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • Function

    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Post-translational
    modifications

    3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Collapsin response mediator protein 2 antibody
    • Collapsin response mediator protein antibody
    • Collapsin response mediator protein hCRMP 2 antibody
    • CRAM antibody
    • CRMP 2 antibody
    • CRMP-2 antibody
    • CRMP2 antibody
    • DHPRP 2 antibody
    • DHPRP2 antibody
    • Dihydropyrimidinase 2 antibody
    • Dihydropyrimidinase like 2 antibody
    • Dihydropyrimidinase like 2 long form antibody
    • Dihydropyrimidinase related protein 2 antibody
    • Dihydropyrimidinase-related protein 2 antibody
    • DPYL 2 antibody
    • DPYL2 antibody
    • DPYL2_HUMAN antibody
    • DPYSL 2 antibody
    • Dpysl2 antibody
    • DRP-2 antibody
    • DRP2 antibody
    • Musunc 33 antibody
    • Musunc33 antibody
    • N2A3 antibody
    • TOAD 64 antibody
    • TOAD64 antibody
    • ULIP 2 protein antibody
    • ULIP-2 antibody
    • Ulip2 antibody
    • Unc-33-like phosphoprotein 2 antibody
    see all

Images

  • All lanes : Anti-CRMP2 antibody (ab62661) at 1/10000 dilution

    Lane 1 : Mouse brain (S1 fraction)
    Lane 2 : Rat brain (S1 fraction)
    Lane 3 : HeLa cells
    Lane 4 : HEK-293T cells transfected with human CRMP2
    Lane 5 : Untransfected HEK-293T cells
    Lane 6 : Mouse brain (S1 fraction): with CRMP2 immunizing peptide at 10 µg/ml

    Secondary
    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate

    Developed using the ECL technique.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

  • All lanes : Anti-CRMP2 antibody (ab62661) at 1/10000 dilution

    Lane 1 : Cell lysate prepared from rat cortex.
    Lane 2 : to 10 : Rat hippocampal homogenates.

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : Alexafluor 680 conjugated mouse polyclonal to rabbit at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa


    Exposure time: 2 minutes

    See Abreview

References

This product has been referenced in:

  • Castillo C  et al. Extracellular Application of CRMP2 Increases Cytoplasmic Calcium through NMDA Receptors. Neuroscience 376:204-223 (2018). Rat . Read more (PubMed: 29555037) »
  • Hori M  et al. PACAP38 differentially effects genes and CRMP2 protein expression in ischemic core and penumbra regions of permanent middle cerebral artery occlusion model mice brain. Int J Mol Sci 15:17014-34 (2014). WB ; Mouse . Read more (PubMed: 25257527) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

If there are many bands on the Western blot, I generally recommend the try to optimize the following steps

I can strongly recommend to test different blocking buffers. Blocking with milk or blocking with BSA can provide very different results (see for example the image on the datasheet of ab9385). If BSA has been used for example as blocking, I would suggest to test also milk blocking.
I can recommend also to maybe dilute the primary antibody further. High antibody concentration can lead to unsprecific staining.
It is also important to know whether the background is might coming from the secondary antibody. This can be checked by performing a “no primary” control, where the Western blot procedure is exactly the same.
In order to be sure that the protein is not in a complex or degraded, I can recommend to use denatured and reduced conditions (by heating the samples prior loading in SDS with DTT or bME) and to prepare the cell lysates freshly and with proteinase inhibitors.
Lastly, maybe the washing steps can be increased.




We are not aware of any specific issues with this antibody. I have therefore provided general tips. Please provide me however also with the protocol you have performed, so I can see whether there is any step for which I could make additional suggestions. To do so, I am attaching our questionnaire which will help you to put the information regarding the samples tested and the protocol used together. Once we receive the completed questionnaire, I will look at the protocol and see if there is any additional suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.

Read More
Application
Western blot
Sample
Rat Cell lysate - whole cell (Hippocampal lysates - mouse)
Loading amount
5 µg
Specification
Hippocampal lysates - mouse
Gel Running Conditions
Reduced Denaturing (10% gel, 5 micrograms protein loaded)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Martin Broadstock

Verified customer

Submitted May 14 2009

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