Overview

  • Product name

    Anti-CRMP2 antibody [EPR7792] - BSA and Azide free
    See all CRMP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7792] to CRMP2 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CRMP2 aa 500-600 (C terminal). The exact sequence is proprietary.
    Database link: Q16555

  • Positive control

    • ICC/IF: NIH/3T3 cells. Flow Cyt: SH-SY5Y and NIH/3T3 cells. WB: PC-12, Jurkat, U-87 MG, HeLa and NIH/3T3 cell lysate. IHC-P: Human astrocytoma, Human colon carcinoma, and Human glioma tissues.
  • General notes

    Ab240952 is the carrier-free version of ab129082. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240952 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 64 kDa (predicted molecular weight: 62 kDa).
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Post-translational
    modifications

    3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Collapsin response mediator protein 2 antibody
    • Collapsin response mediator protein antibody
    • Collapsin response mediator protein hCRMP 2 antibody
    • CRAM antibody
    • CRMP 2 antibody
    • CRMP-2 antibody
    • CRMP2 antibody
    • DHPRP 2 antibody
    • DHPRP2 antibody
    • Dihydropyrimidinase 2 antibody
    • Dihydropyrimidinase like 2 antibody
    • Dihydropyrimidinase like 2 long form antibody
    • Dihydropyrimidinase related protein 2 antibody
    • Dihydropyrimidinase-related protein 2 antibody
    • DPYL 2 antibody
    • DPYL2 antibody
    • DPYL2_HUMAN antibody
    • DPYSL 2 antibody
    • Dpysl2 antibody
    • DRP-2 antibody
    • DRP2 antibody
    • Musunc 33 antibody
    • Musunc33 antibody
    • N2A3 antibody
    • TOAD 64 antibody
    • TOAD64 antibody
    • ULIP 2 protein antibody
    • ULIP-2 antibody
    • Ulip2 antibody
    • Unc-33-like phosphoprotein 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling CRMP2 with Purified ab129082 at 1:1000 dilution (0.12 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)
  • Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling CRMP2 with Purified ab129082 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

  • ab129082 (purified) at 1:20 dilution (0.5µg) immunoprecipitating CRMP2 in U-87 MG whole cell lysate.
    Lane 1 (input): U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab129082 & U-87 MG whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129082 in U-87 MG whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling CRMP2 with Purified ab129082 at 1:1000 dilution (0.12 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)
  • ab129082, at a dilution of 1/100, staining CRMP2 in paraffin embedded Human astrocytoma tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ab129082 staining CRMP2 in NIH/3T3 (mouse embryonic fibroblast) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

  • Overlay histogram showing SH-SY5Y cells stained with ab129082 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129082, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

References

ab240952 has not yet been referenced specifically in any publications.

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