Key features and details
- Rabbit polyclonal to CRMP2 (phospho T509)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CRMP2 (phospho T509) antibody
See all CRMP2 primary antibodies
DescriptionRabbit polyclonal to CRMP2 (phospho T509)
Specificityab192799 detects endogenous levels of CRMP2 only when phosphorylated at Threonine 509.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human CRMP2 (phospho T509) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of threonine 509 (S-V-T(p)-P-K).
Database link: Q16555
- WB: HT29 cell extract; IHC: Human brain tissue.
CRMP-2 is an enzyme with dihydropyrimidinase activity. Plays a role in RhoA-dependent signaling, through interaction with and regulation of Rho kinase. Plays a role in neurogenesis. Aberrantly expressed in fetal Down syndrome brain.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192799 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab192799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 62 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
modifications3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
- Information by UniProt
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
All lanes : Anti-CRMP2 (phospho T509) antibody (ab192799) at 1/500 dilution
Lane 1 : HT29 cell extract
Lane 2 : HT29 cell extract with antigen-specific peptide
Predicted band size: 62 kDa
Immunohistochemical analysis of paraffin-embedded human brain tissue labeling CRMP2 (phospho T509) with ab192799 at 1/50 (left) or ab192799 preincubated with blocking peptide (right).
ab192799 has not yet been referenced specifically in any publications.