Overview

  • Product name

    Anti-CRMP2 (phospho T514) antibody
    See all CRMP2 primary antibodies
  • Description

    Rabbit polyclonal to CRMP2 (phospho T514)
  • Host species

    Rabbit
  • Specificity

    ab62478 detects endogenous levels of CRMP2 only when phosphorylated at threonine 514.
  • Tested applications

    Suitable for: ELISA, ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human CRMP2 around the phosphorylation site of threonine 514 (T-V-TP-P-A).

  • Positive control

    • Human brain tissue and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • Function

    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Post-translational
    modifications

    3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Collapsin response mediator protein 2 antibody
    • Collapsin response mediator protein antibody
    • Collapsin response mediator protein hCRMP 2 antibody
    • CRAM antibody
    • CRMP 2 antibody
    • CRMP-2 antibody
    • CRMP2 antibody
    • DHPRP 2 antibody
    • DHPRP2 antibody
    • Dihydropyrimidinase 2 antibody
    • Dihydropyrimidinase like 2 antibody
    • Dihydropyrimidinase like 2 long form antibody
    • Dihydropyrimidinase related protein 2 antibody
    • Dihydropyrimidinase-related protein 2 antibody
    • DPYL 2 antibody
    • DPYL2 antibody
    • DPYL2_HUMAN antibody
    • DPYSL 2 antibody
    • Dpysl2 antibody
    • DRP-2 antibody
    • DRP2 antibody
    • Musunc 33 antibody
    • Musunc33 antibody
    • N2A3 antibody
    • TOAD 64 antibody
    • TOAD64 antibody
    • ULIP 2 protein antibody
    • ULIP-2 antibody
    • Ulip2 antibody
    • Unc-33-like phosphoprotein 2 antibody
    see all

Images

  • Anti-CRMP2 (phospho T514) antibody (ab62478) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.

  • ab62478, at 1/50 dilution, staining CRMP2 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
  • ab62478, at 1/500 dilution, staining CRMP2 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • Immunofluorescence analysis of RPE cells, staining CRMP2 (phospho T514) with ab62478. Cells were fixed in cold methanol and incubated with primary antibody, followed by an AlexaFluor®488-conjugated anti rabbit IgG.

References

This product has been referenced in:

  • Yang X  et al. Conventional protein kinase Cß-mediated phosphorylation inhibits collapsin response-mediated protein 2 proteolysis and alleviates ischemic injury in cultured cortical neurons and ischemic stroke-induced mice. J Neurochem 137:446-59 (2016). Read more (PubMed: 26788931) »
  • Arbeille E  et al. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis. Nat Commun 6:6366 (2015). Read more (PubMed: 25721514) »
See all 8 Publications for this product

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