Key features and details
- Rabbit polyclonal to CRMP2 (phospho T514)
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CRMP2 (phospho T514) antibody
See all CRMP2 primary antibodies
DescriptionRabbit polyclonal to CRMP2 (phospho T514)
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Orangutan
Synthetic peptide corresponding to Human CRMP2 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human, mouse and rat brain tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab85934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).|
|IP||Use a concentration of 5 µg/ml.|
FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
modifications3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
- Information by UniProt
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
All lanes : Anti-CRMP2 (phospho T514) antibody (ab85934) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
Lane 5 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
Lane 6 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
Lane 7 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
Lane 8 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
Lane 9 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
Lysates/proteins at 10 µg per lane.
Blocking peptides at 1 µg/ml per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62,70 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
The predicted molecular weight of is 62-kDa (SwissProt), however in mouse and rat brain tissue lysates, we observe a band at 70-kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab85934 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85934 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
CRMP2 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to CRMP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab85934.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 70kDa; CRMP2
ab85934 has been referenced in 1 publication.
- Akama K et al. Proteomic identification of differentially expressed genes in neural stem cells and neurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. Biochim Biophys Acta 1814:265-76 (2011). WB ; Monkey . PubMed: 21047566