Product nameAnti-CRMP2 (phospho T514) antibody
See all CRMP2 primary antibodies
DescriptionRabbit polyclonal to CRMP2 (phospho T514)
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Orangutan
Synthetic peptide corresponding to Human CRMP2 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human, mouse and rat brain tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab85934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).|
|IP||Use a concentration of 5 µg/ml.|
FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
modifications3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
- Information by UniProt
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
All lanes : Anti-CRMP2 (phospho T514) antibody (ab85934) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
Lane 5 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
Lane 6 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
Lane 7 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
Lane 8 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
Lane 9 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
Lysates/proteins at 10 µg per lane.
Blocking peptides at 1 µg/ml per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62,70 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
The predicted molecular weight of is 62-kDa (SwissProt), however in mouse and rat brain tissue lysates, we observe a band at 70-kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab85934 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85934 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
CRMP2 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to CRMP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab85934.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 70kDa; CRMP2
This product has been referenced in:
- Akama K et al. Proteomic identification of differentially expressed genes in neural stem cells and neurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. Biochim Biophys Acta 1814:265-76 (2011). WB ; Monkey . Read more (PubMed: 21047566) »