Overview

  • Product name

    Anti-CRMP2 (phospho T514) antibody [EPR7876]
    See all CRMP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7876] to CRMP2 (phospho T514)
  • Host species

    Rabbit
  • Specificity

    ab129066 only detects CRMP2 phosphorylated at Threonine 514.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Threonine 514 of Human CRMP2.

  • Positive control

    • Human fetal brain lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 62 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
    • Post-translational
      modifications

      3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Collapsin response mediator protein 2 antibody
      • Collapsin response mediator protein antibody
      • Collapsin response mediator protein hCRMP 2 antibody
      • CRAM antibody
      • CRMP 2 antibody
      • CRMP-2 antibody
      • CRMP2 antibody
      • DHPRP 2 antibody
      • DHPRP2 antibody
      • Dihydropyrimidinase 2 antibody
      • Dihydropyrimidinase like 2 antibody
      • Dihydropyrimidinase like 2 long form antibody
      • Dihydropyrimidinase related protein 2 antibody
      • Dihydropyrimidinase-related protein 2 antibody
      • DPYL 2 antibody
      • DPYL2 antibody
      • DPYL2_HUMAN antibody
      • DPYSL 2 antibody
      • Dpysl2 antibody
      • DRP-2 antibody
      • DRP2 antibody
      • Musunc 33 antibody
      • Musunc33 antibody
      • N2A3 antibody
      • TOAD 64 antibody
      • TOAD64 antibody
      • ULIP 2 protein antibody
      • ULIP-2 antibody
      • Ulip2 antibody
      • Unc-33-like phosphoprotein 2 antibody
      see all

    Images

    • All lanes : Anti-CRMP2 (phospho T514) antibody [EPR7876] (ab129066) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate, untreated
      Lane 2 : Human fetal brain lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 62 kDa



      The lower panel was analysed using a monoclonal antibody raised against non-phosphoryated CRMP2.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Tian Y  et al. Activation of Transient Receptor Potential Vanilloid 4 Impairs the Dendritic Arborization of Newborn Neurons in the Hippocampal Dentate Gyrus through the AMPK and Akt Signaling Pathways. Front Mol Neurosci 10:190 (2017). WB ; Mouse . Read more (PubMed: 28663724) »
    • Zhang H  et al. Brain-specific Crmp2 deletion leads to neuronal development deficits and behavioural impairments in mice. Nat Commun 7:N/A (2016). WB ; Mouse . Read more (PubMed: 27249678) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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