Key features and details
- Mouse monoclonal [M539] to CRMP2 (phospho T555)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
Product nameAnti-CRMP2 (phospho T555) antibody [M539]
See all CRMP2 primary antibodies
DescriptionMouse monoclonal [M539] to CRMP2 (phospho T555)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Synthetic peptide corresponding to Human CRMP2 (phospho T555). Coupled to carrier protein.
Database link: Q16555
- Rat PC12 cell lysate, stimulated with calyculin A (100 nM).
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
PurityProtein G purified
Purification notesab215742 was affinity purified using CRMP2 (phospho T555) peptide (without carrier).
Our Abpromise guarantee covers the use of ab215742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).|
FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
modifications3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
- Information by UniProt
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
Lanes 1-2 : rabbit anti-CRMP2 (C-terminal region) at 1/500 dilution
Lanes 3-4 : Anti-CRMP2 (phospho T555) antibody [M539] (ab215742) at 1/500 dilution
Lanes 1 & 3 : Rat PC12 cell lysate, stimulated with calyculin A (100 nM)
Lane 2 : Rat PC12 cell lysate, stimulated with calyculin A (100 nM) treated with lambda phosphatase
Lane 4 : Rat PC12 cell lysate, stimulated with calyculin A (100 nM) treated with lambda phosphatase
Predicted band size: 62 kDa
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, and 0.04% Tween20 for 1 h at room temperature.
ab215742 has not yet been referenced specifically in any publications.