• Product name

  • Description

    Rabbit polyclonal to CRMP3
  • Host species

  • Specificity

    No cross reactivity was detected with CRMP1, CRMP2, CRMP4 or CRMP5.
  • Tested applications

    Suitable for: WB, ChIP, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 residue synthetic peptide derived from C-terminal domain of human CRMP3.



Our Abpromise guarantee covers the use of ab36217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 65 kDa.
ChIP Use at an assay dependent concentration. PubMed: 23443259
IHC-Fr 1/500.
IHC-P Use at an assay dependent concentration.


  • Function

    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • Sequence similarities

    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Collapsin response mediator protein 3 antibody
    • CRMP 3 antibody
    • CRMP-3 antibody
    • CRMP3 antibody
    • Dihydropyrimidinase like 4 antibody
    • Dihydropyrimidinase related protein 4 antibody
    • Dihydropyrimidinase-related protein 4 antibody
    • DPYL4_HUMAN antibody
    • DPYSL4 antibody
    • DRP 4 antibody
    • DRP-4 antibody
    • DRP4 antibody
    • ULIP 4 antibody
    • ULIP-4 antibody
    • ULIP4 antibody
    • UNC33 like phosphoprotein 4 antibody
    • UNC33-like phosphoprotein 4 antibody
    see all


  • All lanes : Anti-CRMP3 antibody (ab36217)

    Lane 1 : E.coli transformed with cDNA of human CRMP1
    Lane 2 : E.coli transformed with cDNA of human CRMP2
    Lane 3 : E.coli transformed with cDNA of human CRMP3
    Lane 4 : E.coli transformed with cDNA of human CRMP4
    Lane 5 : E.coli transformed with cDNA of human CRMP5

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • IHC image of ab36217 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36217, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Hou ST  et al. Collapsin response mediator protein 3 deacetylates histone H4 to mediate nuclear condensation and neuronal death. Sci Rep 3:1350 (2013). ChIP ; Mouse . Read more (PubMed: 23443259) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Thank you very much for your reply.

I am very happy to hear the troubleshooting tips were useful.

Please do not hesitate to contact us back for further assistance. Have a very nice day.

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Thank you for taking the time to complete our questionnaire and sending the image. Reviewing this case, I would like to offer some suggestions to help optimise the results from ab36217.

- As a lysis buffer I would suggest using RIPA (radio immuno precipitation assay) buffer due to its higher denaturing level. You can prepare it following the recipe under the next link:


- It could be the membranes are blocked in excess. I would recommend diluting both, the primary and secondary antibody in TBS without milk. You could even try using 5% BSA as a blocking agent instead of milk, which is a milder blocking agent.

I hope these suggestions will help to improve your results. If our troubleshooting tips do not help, please let us know how you would like to proceed and I would be happy to assist you further in resolving the problem you are experiencing.

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with our products.

It is very difficult for us to understand the problem without further details. In order to monitor the product’s quality, and better understand the problem, I would appreciate if you could please send us the attached questionnaire completed. It only takes about 5 minutes to fill out; any images would also be very helpful.

I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your reply.

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of ab36217.

If there is any concrete question you need to know about the specificity of this antibody I will be more than happy to help you.

In any case, all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

All of the information and images we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Unfortunately, we do not have any references or publications available for this particular antibody.

However, this antibody is guaranteed to work in with WB and IHC (in frozen and paraffin embedded sections). If you had any problems using such applications, we would them send you a replacement or reimbursement as guaranteed by the Abpromise guarantee.

I am very sorry I couldn’t help you further. Please do not hesitate to contact us if you need any other advice or information.

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