Product nameAnti-CRMP3 antibody
See all CRMP3 primary antibodies
DescriptionRabbit polyclonal to CRMP3
SpecificityNo cross reactivity was detected with CRMP1, CRMP2, CRMP4 or CRMP5.
Tested applicationsSuitable for: WB, ChIP, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A 15 residue synthetic peptide derived from C-terminal domain of human CRMP3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab36217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 65 kDa.|
|ChIP||Use at an assay dependent concentration. PubMed: 23443259|
|IHC-P||Use at an assay dependent concentration.|
FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Collapsin response mediator protein 3 antibody
- CRMP 3 antibody
- CRMP-3 antibody
All lanes : Anti-CRMP3 antibody (ab36217)
Lane 1 : E.coli transformed with cDNA of human CRMP1
Lane 2 : E.coli transformed with cDNA of human CRMP2
Lane 3 : E.coli transformed with cDNA of human CRMP3
Lane 4 : E.coli transformed with cDNA of human CRMP4
Lane 5 : E.coli transformed with cDNA of human CRMP5
Predicted band size: 65 kDa
Observed band size: 65 kDa
IHC image of ab36217 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36217, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.