Anti-CRMP4 antibody (ab244319)
Key features and details
- Rabbit polyclonal to CRMP4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CRMP4 antibody
See all CRMP4 primary antibodies -
Description
Rabbit polyclonal to CRMP4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human CRMP4 aa 100-250.
Database link: Q14195 -
Positive control
- IHC-P: Human smooth muscle tissue. WB: NTERA-2 cl.D1 [NT2/D1] and PC-12 cell lysate. ICC/IF: U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. -
Tissue specificity
Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord. -
Sequence similarities
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
Post-translational
modificationsPhosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3. -
Cellular localization
Cytoplasm. Cell projection > growth cone. Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone. - Information by UniProt
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Database links
- Entrez Gene: 1809 Human
- Entrez Gene: 22240 Mouse
- Entrez Gene: 25418 Rat
- Omim: 601168 Human
- SwissProt: Q14195 Human
- SwissProt: Q62188 Mouse
- SwissProt: Q62952 Rat
- Unigene: 519659 Human
see all -
Alternative names
- Collapsin response mediator protein 4 antibody
- Collapsin response mediator protein 4 long antibody
- CRMP 4 antibody
see all
Images
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PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling CRMP4 using ab244319 at 4 µg/ml (green) in ICC/IF.
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Formalin-fixed, paraffin-embedded human smooth muscle tissue stained for CRMP4 with ab244319 at a 1/1000 dilution in immunohistochemical analysis.
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All lanes : Anti-CRMP4 antibody (ab244319) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate -
Anti-CRMP4 antibody (ab244319) at 0.4 µg/ml + NTERA-2 cl.D1 [NT2/D1] (Human malignant pluripotent embryonic carcinoma cell line) cell lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab244319 has been referenced in 1 publication.
- Lai S et al. The impact of CRMP4 SUMOylation on the Cav1.2 interaction, neurite outgrowth and thermal pain sensitivity. J Integr Neurosci 20:595-603 (2021). PubMed: 34645092