Overview

  • Product name
  • Description
    Rabbit polyclonal to CRMP4
  • Host species
    Rabbit
  • Specificity
    No cross reactivity was detected with CRMP1, CRMP2, CRMP3 or CRMP5.
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 residue synthetic peptide derived from C-terminal domain of human collapsing-response mediator (Type 4).

Properties

Applications

Our Abpromise guarantee covers the use of ab36204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 65 kDa.
IHC-Fr 1/500.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • Tissue specificity
    Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.
  • Sequence similarities
    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Post-translational
    modifications
    Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.
  • Cellular localization
    Cytoplasm. Cell projection > growth cone. Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone.
  • Information by UniProt
  • Database links
  • Alternative names
    • Collapsin response mediator protein 4 antibody
    • Collapsin response mediator protein 4 long antibody
    • CRMP 4 antibody
    • CRMP-4 antibody
    • CRMP4 antibody
    • Dihydropyrimidinase like 3 antibody
    • Dihydropyrimidinase related protein 3 antibody
    • Dihydropyrimidinase-related protein 3 antibody
    • DPYL3_HUMAN antibody
    • DPYSL3 antibody
    • DRP 3 antibody
    • DRP-3 antibody
    • DRP3 antibody
    • LCRMP antibody
    • TUC4 antibody
    • ULIP 1 antibody
    • ULIP antibody
    • ULIP-1 antibody
    • ULIP1 antibody
    • UNc 33 like phosphoprotein 1 antibody
    • Unc 33 like phosphoprotein antibody
    • Unc-33-like phosphoprotein 1 antibody
    see all

Images

  • All lanes : Anti-CRMP4 antibody (ab36204)

    Lane 1 : E.coli lysate transformed with cDNA of human CRMP1
    Lane 2 : E.coli lysate transformed with cDNA of human CRMP2
    Lane 3 : E.coli lysate transformed with cDNA of human CRMP3
    Lane 4 : E.coli lysate transformed with cDNA of human CRMP4
    Lane 5 : E.coli lysate transformed with cDNA of human CRMP5

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

References

This product has been referenced in:
  • Yoneda A  et al. A collapsin response mediator protein 2 isoform controls myosin II-mediated cell migration and matrix assembly by trapping ROCK II. Mol Cell Biol 32:1788-804 (2012). Read more (PubMed: 22431514) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, I can provide a range of the IgG: 10-15 mg/ml.

Isotype controls:



can be searched at this site: https://www.abcam.com/index.html?c=718

ab36204, ab48614, ab66219, ab102998 --> Rabbit IgG - ab37406, ab125938, ab128142

ab83508 --> Mouse IgG1 - ab91353, ab27479, ab81032



Positive control:



ab36204 - CRMP4 - It is mainly expressed in heart and skeletal muscle and also strongly expressed in fetal brain and spinal cord (human, mouse or rat),

ab48614 --Ceruloplasmin - It is expressed by the liver and secreted in plasma,

ab66219 -- Oprl1 - Brain (cortex),

ab83508 -- NG2 - It is detected only in malignant melanoma cells, SK-MEL-28 cells,

ab102998 --B3GNT5- only tested in WB - Widely expressed. It is highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum, MDA-MB435 cell line

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for your enquiry and your interest in our products.

This is to confirm that we can only provide the concentration for affinity purified antibodies. For whole serum or supernatant we do not quantify the IgG concentration. I have contacted the lab to get the requested information for you concerning ab66219 (Lot: GR9812).

Would you be so kind to clarify if you wish to look for isotype control or positive control for these primary antibodies listed below?

ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> Rabbit IgG
ab83508 --> Mouse IgG1
ab102998 --> Rabbit IgG

I look forward to hearing from you soon. As soon as I have further information on the concentration (ab66219), I will contact you again.

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Answer

My colleague Teresa has forwarded your request to our team.

I have checked the datasheets for the PFA- perfusion fixed frozen section application (IHC-FoFr), andthe antibodiesare all not tested for this. This means unfortunately, that we cannot guarantee the outcome.

However,I can offer a discount off a future purchase if you buyab36204, ab66219, ab83508and /or ab102998now, test it in IHC-FoFr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 freeprimary antibodyper tested antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab36204, ab66219, ab83508 and /or ab102998 in IHC-FoFr. I will then send a discount code. This code must be issued before purchasing ab36204, ab66219, ab83508 and /or ab102998 so please wait for my reply before ordering.

2. Purchase ab36204, ab66219, ab83508 and /or ab102998 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-FoFr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab36204, ab66219, ab83508 and /or ab102998 turns out to be unsuitable for IHC-FoFr, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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