Question (42434) | Anti-CRMP4 antibody (ab36204)

Go to datasheet (ab36204)

Question

Dear Sir or Madam,
We recently bought the following antibodies from your company: ab36204; ab48614; ab66219; ab83508; ab102998. As we would like to test at least four of them on 4%PFA, sucrose fixed brain tissue and include the correct controls I have a few questions:
I received the following list:
ab36204 (Lot: GR42382) --> concentration: N/A 100µl
ab48614 (Lot: GR62244) --> concentration: 0,7699 mg/ml (100µg)
ab66219 (Lot: GR9812) --> concentration: N/A 100µl
ab83508 (Lot: GR38090) --> concentration: 0.5 mg/ml (100µg)
ab102998 (Lot: GR80395) --> concentration 0.25mg/ml (100µg)
In order to include a control with the same antibody concentration as for the primary antibody it would be good to know the concentration of ab36204 and ab66219, too. Could you help me concerning that matter?
Second question, do you have any experience with good control antibodies? I thought the following:
ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> Rabbit IgG
ab83508 --> Mouse IgG1
ab102998 --> Rabbit IgG
I think that is also what you suggested in the data sheets, I just would like to make sure, that I am correct.
I would be very happy about a rapid answer.
Best regards,

Answer

Thank you for your enquiry and your interest in our products.

This is to confirm that we can only provide the concentration for affinity purified antibodies. For whole serum or supernatant we do not quantify the IgG concentration. I have contacted the lab to get the requested information for you concerning ab66219 (Lot: GR9812).

Would you be so kind to clarify if you wish to look for isotype control or positive control for these primary antibodies listed below?

ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> Rabbit IgG
ab83508 --> Mouse IgG1
ab102998 --> Rabbit IgG

I look forward to hearing from you soon. As soon as I have further information on the concentration (ab66219), I will contact you again.

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