Recombinant Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
![Western blot - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)](https://www.abcam.com/ps/products/126/ab126787/Images/CRMP4-Primary-antibodies-ab126787-1.JPG "Western blot - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7593(B)] to CRMP4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CRMP4 antibody [EPR7593(B)]
See all CRMP4 primary antibodies -
Description
Rabbit monoclonal [EPR7593(B)] to CRMP4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A synthetic peptide, corresponding to residues in Human CRMP4 (UniProt Q14195).
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Positive control
- Human hippocampus and fetal brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.50 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7593(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
Target
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Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. -
Tissue specificity
Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord. -
Sequence similarities
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
Post-translational
modificationsPhosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3. -
Cellular localization
Cytoplasm. Cell projection > growth cone. Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone. - Information by UniProt
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Database links
- Entrez Gene: 1809 Human
- Entrez Gene: 22240 Mouse
- Entrez Gene: 25418 Rat
- Omim: 601168 Human
- SwissProt: Q14195 Human
- SwissProt: Q62188 Mouse
- SwissProt: Q62952 Rat
- Unigene: 519659 Human
see all -
Alternative names
- Collapsin response mediator protein 4 antibody
- Collapsin response mediator protein 4 long antibody
- CRMP 4 antibody
see all
Images
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Western blot - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)All lanes : Anti-CRMP4 antibody [EPR7593(B)] (ab126787) at 1/1000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa -
![OI-RD Scanning - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)](https://www.abcam.com/ps/products/126/ab126787/Images/ab126787-140856-anti-crmp4-antibody-epr7593b.jpg)
OI-RD Scanning - Anti-CRMP4 antibody [EPR7593(B)] (ab126787) -
![Anti-CRMP4 antibody [EPR7593(B)] (ab126787)](https://www.abcam.com/ps/products/126/ab126787/Images/ab126787-4-benefits-of-recombinant-antibodies.png)
Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
Protocols
Datasheets and documents
References (1)
ab126787 has been referenced in 1 publication.
- Wang Z et al. Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide-induced mouse model of depression. Mol Med Rep 13:1813-20 (2016). PubMed: 26718926
