Recombinant
RabMAb

Recombinant Anti-CRMP4 antibody [EPR7593(B)] (ab126787)

Reviews (2)Q&A (1)

Overview

  • Product name

    Anti-CRMP4 antibody [EPR7593(B)]
    See all CRMP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR7593(B)] to CRMP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    A synthetic peptide, corresponding to residues in Human CRMP4 (UniProt Q14195).

  • Positive control

    • Human hippocampus and fetal brain lysates.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

    • Tissue specificity

      Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.

    • Sequence similarities

      Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.

    • Post-translational
      modifications

      Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.

    • Cellular localization

      Cytoplasm. Cell projection > growth cone. Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Collapsin response mediator protein 4 antibody
      • Collapsin response mediator protein 4 long antibody
      • CRMP 4 antibody
      • CRMP-4 antibody
      • CRMP4 antibody
      • Dihydropyrimidinase like 3 antibody
      • Dihydropyrimidinase related protein 3 antibody
      • Dihydropyrimidinase-related protein 3 antibody
      • DPYL3_HUMAN antibody
      • DPYSL3 antibody
      • DRP 3 antibody
      • DRP-3 antibody
      • DRP3 antibody
      • LCRMP antibody
      • TUC4 antibody
      • ULIP 1 antibody
      • ULIP antibody
      • ULIP-1 antibody
      • ULIP1 antibody
      • UNc 33 like phosphoprotein 1 antibody
      • Unc 33 like phosphoprotein antibody
      • Unc-33-like phosphoprotein 1 antibody
      see all

    Images

    • Western blot - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      Western blot - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      All lanes : Anti-CRMP4 antibody [EPR7593(B)] (ab126787) at 1/1000 dilution

      Lane 1 : Human hippocampus lysate
      Lane 2 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 62 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      Immunocytochemistry/ Immunofluorescence - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)This image is courtesy of an Abreview submitted by Radhi Praba Velayutham

      ab126787 staining CRMP4 in Human medulloblasma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone mixture, permeabilized with 0.25% Triton X-100 in PBS and blocked with 5% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBS + 5% Goat serum) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

       

      See Abreview

    • Other - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      Other - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126787 has not yet been referenced specifically in any publications.

    Publishing research using ab126787? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-CRMP4 antibody [EPR7593(B)]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (9%)
    Sample
    Human Cell lysate - whole cell (human medulloblastoma cell line)
    Specification
    human medulloblastoma cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Ms. Radhi Praba Velayutham

    Verified customer

    Submitted Nov 13 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CRMP4 antibody [EPR7593(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Human Cell (human medulloblastoma cellline)
    Specification
    human medulloblastoma cellline
    Permeabilization
    Yes - PBS-0.25% Triton-X100
    Fixative
    acetone:methanol mixture
    Read More

    Ms. Radhi Praba Velayutham

    Verified customer

    Submitted Oct 31 2013

    Question



    I am planning to order an abcam antibody for CRMP-4 protein. But, I do no know whether this antibody will recognize both iso-forms of this gene.

    Read More
    Answer

    Thank you for contacting us. This antibody should detect both isofrms of the protein.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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