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RecombinantRabMAb

Recombinant Anti-CRMP4 antibody [EPR7593(B)] (ab126787)

  • Datasheet
Reviews (2)Q&A (1)References (1)

Product price, shipping and contact information

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Abpromise

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Find out more.

Western blot - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
  • OI-RD Scanning - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
  • Anti-CRMP4 antibody [EPR7593(B)] (ab126787)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7593(B)] to CRMP4
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Protein
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Overview

  • Product name

    Anti-CRMP4 antibody [EPR7593(B)]
    See all CRMP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR7593(B)] to CRMP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide, corresponding to residues in Human CRMP4 (UniProt Q14195).

  • Positive control

    • Human hippocampus and fetal brain lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 7.50 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7593(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Neurology process
    • Neurogenesis

Associated products

  • Alternative Versions

    • Anti-CRMP4 antibody [EPR7593(B)] - BSA and Azide free (ab248183)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CRMP4 protein (ab132289)

Applications

Our Abpromise guarantee covers the use of ab126787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
    • Tissue specificity

      Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.
    • Sequence similarities

      Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
    • Post-translational
      modifications

      Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.
    • Cellular localization

      Cytoplasm. Cell projection > growth cone. Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone.
    • Target information above from: UniProt accession Q14195 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1809 Human
      • Entrez Gene: 22240 Mouse
      • Entrez Gene: 25418 Rat
      • Omim: 601168 Human
      • SwissProt: Q14195 Human
      • SwissProt: Q62188 Mouse
      • SwissProt: Q62952 Rat
      • Unigene: 519659 Human
      • Unigene: 428551 Mouse
      • Unigene: 8180 Mouse
      • Unigene: 93365 Rat
      see all
    • Alternative names

      • Collapsin response mediator protein 4 antibody
      • Collapsin response mediator protein 4 long antibody
      • CRMP 4 antibody
      • CRMP-4 antibody
      • CRMP4 antibody
      • Dihydropyrimidinase like 3 antibody
      • Dihydropyrimidinase related protein 3 antibody
      • Dihydropyrimidinase-related protein 3 antibody
      • DPYL3_HUMAN antibody
      • DPYSL3 antibody
      • DRP 3 antibody
      • DRP-3 antibody
      • DRP3 antibody
      • LCRMP antibody
      • TUC4 antibody
      • ULIP 1 antibody
      • ULIP antibody
      • ULIP-1 antibody
      • ULIP1 antibody
      • UNc 33 like phosphoprotein 1 antibody
      • Unc 33 like phosphoprotein antibody
      • Unc-33-like phosphoprotein 1 antibody
      see all

    Images

    • Western blot - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      Western blot - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      All lanes : Anti-CRMP4 antibody [EPR7593(B)] (ab126787) at 1/1000 dilution

      Lane 1 : Human hippocampus lysate
      Lane 2 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 62 kDa

    • OI-RD Scanning - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      OI-RD Scanning - Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
      Anti-CRMP4 antibody [EPR7593(B)] (ab126787)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab126787? Please let us know so that we can cite the reference in this datasheet.

    ab126787 has been referenced in 1 publication.

    • Wang Z  et al. Proteomic analysis reveals energy metabolic dysfunction and neurogenesis in the prefrontal cortex of a lipopolysaccharide-induced mouse model of depression. Mol Med Rep 13:1813-20 (2016). PubMed: 26718926

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-CRMP4 antibody [EPR7593(B)]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (9%)
    Sample
    Human Cell lysate - whole cell (human medulloblastoma cell line)
    Specification
    human medulloblastoma cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Ms. Radhi Praba Velayutham

    Verified customer

    Submitted Nov 13 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CRMP4 antibody [EPR7593(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Human Cell (human medulloblastoma cellline)
    Specification
    human medulloblastoma cellline
    Permeabilization
    Yes - PBS-0.25% Triton-X100
    Fixative
    acetone:methanol mixture
    Read More

    Ms. Radhi Praba Velayutham

    Verified customer

    Submitted Oct 31 2013

    Question



    I am planning to order an abcam antibody for CRMP-4 protein. But, I do no know whether this antibody will recognize both iso-forms of this gene.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 20 2013

    Answer

    Thank you for contacting us. This antibody should detect both isofrms of the protein.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 20 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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