Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13127(B)] to CRMP5
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-CRMP5 antibody [EPR13127(B)]
See all CRMP5 primary antibodies
DescriptionRabbit monoclonal [EPR13127(B)] to CRMP5
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CRMP5 aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BPU6
- Fetal brain lysate, Human cerebellum lysate, SH-SY5Y lysates, paraffin-embedded Human brain tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 61 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
- Information by UniProt
- Collapsin response mediator protein 5 antibody
- CRAM antibody
- CRMP 3 associated molecule antibody
All lanes : Anti-CRMP5 antibody [EPR13127(B)] (ab176331) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling CRMP5 with ab176331 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab176331 at 1/10 dilution.
ab176331 has not yet been referenced specifically in any publications.