Overview

  • Product name

    Anti-CRNKL1 antibody [EPR16064]
    See all CRNKL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16064] to CRNKL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CRNKL1 aa 750 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BZJ0

  • Positive control

    • Human seminoma lysate; Human testis tissue; Rat kidney tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16064
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
WB 1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P 1/300 - 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Involved in pre-mRNA splicing process.
  • Tissue specificity

    Widely expressed. Highly expressed in testis. Not expressed in brain and lung.
  • Sequence similarities

    Belongs to the crooked-neck family.
    Contains 17 HAT repeats.
  • Cellular localization

    Nucleus speckle. Cytoplasm. Colocalizes with core spliceosomal snRNP proteins. Also diffusely expressed in cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1200013P10Rik antibody
    • 5730590A01Rik antibody
    • C80326 antibody
    • CLF antibody
    • Clf1 antibody
    • CRN antibody
    • Crn crooked neck like 1 (Drosophila) antibody
    • Crnkl1 antibody
    • CRNL1_HUMAN antibody
    • Crooked neck homolog antibody
    • Crooked neck like protein 1 antibody
    • Crooked neck pre mRNA splicing factor 1 antibody
    • Crooked neck-like protein 1 antibody
    • hCrn antibody
    • MGC55327 antibody
    • MGC93283 antibody
    • MSTP021 antibody
    • SYF3 antibody
    • SYF3 pre mRNA splicing factor antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling CRNKL1 with ab192248 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

    The two negative controls are ab192248 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

  • Anti-CRNKL1 antibody [EPR16064] (ab192248) at 1/20000 dilution + Human seminoma lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 100 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab192248 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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