RecombinantRabMAb

Recombinant Anti-CRNKL1 antibody [EPR16064] (ab192248)

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Immunocytochemistry/ Immunofluorescence - Anti-CRNKL1 antibody [EPR16064] (ab192248)
  • Western blot - Anti-CRNKL1 antibody [EPR16064] (ab192248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)
  • Anti-CRNKL1 antibody [EPR16064] (ab192248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16064] to CRNKL1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CRNKL1 antibody [EPR16064]
    See all CRNKL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16064] to CRNKL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CRNKL1 aa 750 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BZJ0

  • Positive control

    • Human seminoma lysate; Human testis tissue; Rat kidney tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16064

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
WB 1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P 1/300 - 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Involved in pre-mRNA splicing process.

  • Tissue specificity

    Widely expressed. Highly expressed in testis. Not expressed in brain and lung.

  • Sequence similarities

    Belongs to the crooked-neck family.
    Contains 17 HAT repeats.

  • Cellular localization

    Nucleus speckle. Cytoplasm. Colocalizes with core spliceosomal snRNP proteins. Also diffusely expressed in cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 1200013P10Rik antibody
    • 5730590A01Rik antibody
    • C80326 antibody
    • CLF antibody
    • Clf1 antibody
    • CRN antibody
    • Crn crooked neck like 1 (Drosophila) antibody
    • Crnkl1 antibody
    • CRNL1_HUMAN antibody
    • Crooked neck homolog antibody
    • Crooked neck like protein 1 antibody
    • Crooked neck pre mRNA splicing factor 1 antibody
    • Crooked neck-like protein 1 antibody
    • hCrn antibody
    • MGC55327 antibody
    • MGC93283 antibody
    • MSTP021 antibody
    • SYF3 antibody
    • SYF3 pre mRNA splicing factor antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Immunocytochemistry/ Immunofluorescence - Anti-CRNKL1 antibody [EPR16064] (ab192248)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling CRNKL1 with ab192248 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

    The two negative controls are ab192248 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

  • Western blot - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Western blot - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Anti-CRNKL1 antibody [EPR16064] (ab192248) at 1/20000 dilution + Human seminoma lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 100 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Anti-CRNKL1 antibody [EPR16064] (ab192248)

References (0)

Publishing research using ab192248? Please let us know so that we can cite the reference in this datasheet.

ab192248 has not yet been referenced specifically in any publications.

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