Anti-Rootletin antibody (ab121653)
Key features and details
- Rabbit polyclonal to Rootletin
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Rootletin antibody -
Description
Rabbit polyclonal to Rootletin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Rootletin aa 406-483.
Sequence:VKRLEKQNLEKDQVNKDLTEKLEALESLRLQEQAALETEDGEGLQQTLRD LAQAVLSDSESGVQLSGSERTADASNGS
-
Positive control
- IHC-P: Human fallopian tube, bronchus, liver, cerebral cortex and testis tissue; ICC: U2OS cells; WB: SCLC-21H.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 229 kDa.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
|
|
ICC |
Use a concentration of 0.25 - 2 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 229 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
ICC
Use a concentration of 0.25 - 2 µg/ml. |
Target
-
Function
Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis. -
Sequence similarities
Belongs to the rootletin family. -
Post-translational
modificationsPhosphorylated by NEK2 which may regulate its association with centrosomes. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase. - Information by UniProt
-
Database links
- Entrez Gene: 9696 Human
- SwissProt: Q5TZA2 Human
- Unigene: 309403 Human
-
Alternative names
- ciliary rootlet coiled coil antibody
- Ciliary rootlet coiled-coil protein antibody
- Crocc antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchus tissue labelling Rootletin with ab121653 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunocytochemistry analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cells labelling Rootletin with ab121653 at 2 µg/mL.
-
Anti-Rootletin antibody (ab121653) at 0.4 µg/ml + SCLC-21H (human small cell lung carcinoma cell line) cell lysate
Predicted band size: 229 kDa -
ab121653, at 1/150 dilution, staining Rootletin in Human fallopian tube tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
-
Immunohistochemical analysis of human testis tissue labeling Rootletin with ab121653 at 1/50 dilution.
-
Immunohistochemical analysis of human liver tissue labeling Rootletin with ab121653 at 1/50 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab121653 has been referenced in 1 publication.
- Saade M et al. Multimerization of Zika Virus-NS5 Causes Ciliopathy and Forces Premature Neurogenesis. Cell Stem Cell 27:920-936.e8 (2020). PubMed: 33147489