Anti-CROT/COT antibody (ab175450)
Key features and details
- Goat polyclonal to CROT/COT
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-CROT/COT antibody
See all CROT/COT primary antibodies -
Description
Goat polyclonal to CROT/COT -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog -
Immunogen
Synthetic peptide corresponding to Rat CROT/COT aa 178-199 (internal sequence). NP_114193.1.
Sequence:NYFKTESEGHCPTH
Database link: P11466 -
Positive control
- Mouse liver lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab175450 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 70 kDa.
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 70 kDa. 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. -
Cellular localization
Peroxisome. - Information by UniProt
-
Database links
- Entrez Gene: 482283 Dog
- Entrez Gene: 54677 Human
- Entrez Gene: 74114 Mouse
- Entrez Gene: 83842 Rat
- Omim: 606090 Human
- SwissProt: Q9UKG9 Human
- SwissProt: Q9DC50 Mouse
- SwissProt: P11466 Rat
see all -
Alternative names
- Carnitine O octanoyltransferase antibody
- COT antibody
- CROT antibody
see all
Images
Datasheets and documents
-
Datasheet download
References (2)
ab175450 has been referenced in 2 publications.
- Wang JL et al. Mesenchymal stem cells ameliorate lipid metabolism through reducing mitochondrial damage of hepatocytes in the treatment of post-hepatectomy liver failure. Cell Death Dis 12:111 (2021). PubMed: 33479191
- Ni Y et al. LncRNA EGOT regulates the proliferation and apoptosis of colorectal cancer by miR-33b-5p/CROT axis. Biosci Rep N/A:N/A (2020). PubMed: 32373939