Recombinant
RabMAb

Recombinant Anti-CRP-1 antibody [EPR14345] (ab183029)

Overview

  • Product name

    Anti-CRP-1 antibody [EPR14345]
    See all CRP-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14345] to CRP-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CRP-1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P50238

  • Positive control

    • T47D, THP1 and HepG2 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
IP 1/20 - 1/40.

Target

  • Function

    Seems to have a role in zinc absorption and may function as an intracellular zinc transport protein.
  • Sequence similarities

    Contains 1 LIM zinc-binding domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • CRHP antibody
    • CRIP antibody
    • CRIP1 antibody
    • CRIP1_HUMAN antibody
    • CRP-1 antibody
    • CRP1 antibody
    • Cysteine rich heart protein antibody
    • Cysteine rich intestinal protein antibody
    • Cysteine rich protein 1 (intestinal) antibody
    • Cysteine rich protein 1 antibody
    • Cysteine-rich heart protein antibody
    • Cysteine-rich intestinal protein antibody
    • Cysteine-rich protein 1 antibody
    • FLJ40971 antibody
    • hCRHP antibody
    see all

Images

  • Western blot analysis of immunoprecipitation pellet from THP1 cell lysate immunoprecipitated using ab183029 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • All lanes : Anti-CRP-1 antibody [EPR14345] (ab183029) at 1/20000 dilution

    Lane 1 : T47D cell lysate
    Lane 2 : THP1 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 9 kDa

References

ab183029 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain homogenate)
Gel Running Conditions
Reduced Denaturing (Tricine gel 10-20%)
Loading amount
10 µg
Specification
Brain homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 29 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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