Product nameAnti-CRP-2 antibody
See all CRP-2 primary antibodies
DescriptionRabbit polyclonal to CRP-2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- IHC-P: Human placenta tissue. WB: RT4 and U-251 MG sp cell lysates; Human liver and tonsil lysates. ICC/IF: U-2 OS cells.
Protein previously labeled as CRIP2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||1/100 - 1/250. Predicted molecular weight: 22 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityWidespread tissue expression; highest levels in the heart.
Sequence similaritiesContains 2 LIM zinc-binding domains.
- Information by UniProt
- CRIP 2 antibody
- CRIP antibody
- CRIP2 antibody
All lanes : Anti-CRP-2 antibody (ab224563) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 22 kDa
Paraffin-embedded human placenta tissue stained for CRP-2 using ab224563 at 1/500 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for CRP-2 (green) using ab224563 at 4 µg/ml in ICC/IF.
ab224563 has not yet been referenced specifically in any publications.