Overview

  • Product name

    Anti-CRSP2 / MED14 antibody [2214C1a]
    See all CRSP2 / MED14 primary antibodies
  • Description

    Mouse monoclonal [2214C1a] to CRSP2 / MED14
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant human CRSP2/ MED14 fragment

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Ab51349 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
  • Clonality

    Monoclonal
  • Clone number

    2214C1a
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 161 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the Mediator complex subunit 14 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator-recruited cofactor 150 kDa component antibody
    • ARC150 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 2 antibody
    • CRSP complex subunit 2 antibody
    • DRIP150 antibody
    • EXLM1 antibody
    • hRGR1 antibody
    • MED14 antibody
    • MED14_HUMAN antibody
    • Mediator complex subunit 14 antibody
    • Mediator of RNA polymerase II transcription subunit 14 antibody
    • RGR1 antibody
    • RGR1 homolog antibody
    • Thyroid hormone receptor-associated protein complex 170 kDa component antibody
    • Transcriptional coactivator CRSP150 antibody
    • Trap170 antibody
    • Vitamin D3 receptor-interacting protein complex 150 kDa component antibody
    see all

Images

References

This product has been referenced in:

  • Englert NA  et al. Epigenetic modification of histone 3 lysine 27: mediator subunit MED25 is required for the dissociation of polycomb repressive complex 2 from the promoter of cytochrome P450 2C9. J Biol Chem 290:2264-78 (2015). Read more (PubMed: 25391650) »
See 1 Publication for this product

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