Key features and details
- Mouse monoclonal [2214C1a] to CRSP2 / MED14
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CRSP2 / MED14 antibody [2214C1a]
See all CRSP2 / MED14 primary antibodies
DescriptionMouse monoclonal [2214C1a] to CRSP2 / MED14
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant human CRSP2/ MED14 fragment
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, PBS
Concentration information loading...
PurityProtein G purified
Purification notesAb51349 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
Our Abpromise guarantee covers the use of ab51349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 161 kDa.|
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similaritiesBelongs to the Mediator complex subunit 14 family.
- Information by UniProt
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349) + Recombinant CRSP2/MED14
Predicted band size: 161 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
ab51349 has been referenced in 1 publication.
- Englert NA et al. Epigenetic modification of histone 3 lysine 27: mediator subunit MED25 is required for the dissociation of polycomb repressive complex 2 from the promoter of cytochrome P450 2C9. J Biol Chem 290:2264-78 (2015). PubMed: 25391650