Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349)

Submit a review Submit a question References (1)

Key features and details

  • Mouse monoclonal [2214C1a] to CRSP2 / MED14
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CRSP2 / MED14 antibody [2214C1a]
    See all CRSP2 / MED14 primary antibodies

  • Description

    Mouse monoclonal [2214C1a] to CRSP2 / MED14

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant human CRSP2/ MED14 fragment

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Ab51349 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.

  • Clonality

    Monoclonal

  • Clone number

    2214C1a

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 161 kDa.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the Mediator complex subunit 14 family.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activator-recruited cofactor 150 kDa component antibody
      • ARC150 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 2 antibody
      • CRSP complex subunit 2 antibody
      • DRIP150 antibody
      • EXLM1 antibody
      • hRGR1 antibody
      • MED14 antibody
      • MED14_HUMAN antibody
      • Mediator complex subunit 14 antibody
      • Mediator of RNA polymerase II transcription subunit 14 antibody
      • RGR1 antibody
      • RGR1 homolog antibody
      • Thyroid hormone receptor-associated protein complex 170 kDa component antibody
      • Transcriptional coactivator CRSP150 antibody
      • Trap170 antibody
      • Vitamin D3 receptor-interacting protein complex 150 kDa component antibody
      see all

    Images

    References (1)

    Publishing research using ab51349? Please let us know so that we can cite the reference in this datasheet.

    ab51349 has been referenced in 1 publication.

    • Englert NA  et al. Epigenetic modification of histone 3 lysine 27: mediator subunit MED25 is required for the dissociation of polycomb repressive complex 2 from the promoter of cytochrome P450 2C9. J Biol Chem 290:2264-78 (2015). PubMed: 25391650

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab51349.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com