Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-CRSP2 / MED14 antibody [2214C1a]
See all CRSP2 / MED14 primary antibodies -
DescriptionMouse monoclonal [2214C1a] to CRSP2 / MED14
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Host speciesMouse
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Tested applicationsSuitable for: WB, Dot blotmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant human CRSP2/ MED14 fragment
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4). -
Concentration information loading... -
PurityProtein G purified
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Purification notesAb51349 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
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ClonalityMonoclonal
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Clone number2214C1a
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab51349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. Predicted molecular weight: 161 kDa. | |
| Dot blot | Use at an assay dependent dilution. |
Target
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FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the Mediator complex subunit 14 family.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 9282 Human
- Omim: 300182 Human
- SwissProt: O60244 Human
- Unigene: 407604 Human
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Alternative names
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
see all
Images
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![Western blot - Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349)](https://www.abcam.com/ps/products/51/ab51349/Images/ab51349.gif)
Western blot - Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349)Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349) + Recombinant CRSP2/MED14
Predicted band size: 161 kDa
Observed band size: 36 kDa (why is the actual band size different from the predicted?)
Protocols
References
This product has been referenced in:
- Englert NA et al. Epigenetic modification of histone 3 lysine 27: mediator subunit MED25 is required for the dissociation of polycomb repressive complex 2 from the promoter of cytochrome P450 2C9. J Biol Chem 290:2264-78 (2015). Read more (PubMed: 25391650) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"