Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CRSP2 / MED14 antibody [2214C1a]
See all CRSP2 / MED14 primary antibodies -
Description
Mouse monoclonal [2214C1a] to CRSP2 / MED14 -
Host species
Mouse -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human CRSP2/ MED14 fragment
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Ab51349 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2214C1a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab51349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. Predicted molecular weight: 161 kDa. | |
| Dot blot | Use at an assay dependent dilution. |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the Mediator complex subunit 14 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9282 Human
- Omim: 300182 Human
- SwissProt: O60244 Human
- Unigene: 407604 Human
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Alternative names
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
see all
Images
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![Western blot - Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349)](https://www.abcam.com/ps/products/51/ab51349/Images/ab51349-63377-anti-crsp2-med14-antibody-2214c1a-western-blot.gif)
Western blot - Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349)Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349) + Recombinant CRSP2/MED14
Predicted band size: 161 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Englert NA et al. Epigenetic modification of histone 3 lysine 27: mediator subunit MED25 is required for the dissociation of polycomb repressive complex 2 from the promoter of cytochrome P450 2C9. J Biol Chem 290:2264-78 (2015). Read more (PubMed: 25391650) »
