Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349)

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Western blot - Anti-CRSP2 / MED14 antibody [2214C1a] (ab51349)

    Key features and details

    • Mouse monoclonal [2214C1a] to CRSP2 / MED14
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG1

    Overview

    • Product name

      Anti-CRSP2 / MED14 antibody [2214C1a]
      See all CRSP2 / MED14 primary antibodies

    • Description

      Mouse monoclonal [2214C1a] to CRSP2 / MED14

    • Host species

      Mouse

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Recombinant fragment

    • Immunogen

      Recombinant human CRSP2/ MED14 fragment

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • Storage buffer

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, PBS
    • Concentration information loading...

    • Purity

      Protein G purified

    • Purification notes

      Ab51349 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.

    • Clonality

      Monoclonal

    • Clone number

      2214C1a

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab51349 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 161 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 161 kDa.

    Target

    • Function

      Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the Mediator complex subunit 14 family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Alternative names

      • Activator-recruited cofactor 150 kDa component antibody
      • ARC150 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 2 antibody
      • CRSP complex subunit 2 antibody
      • DRIP150 antibody
      • EXLM1 antibody
      • hRGR1 antibody
      • MED14 antibody
      • MED14_HUMAN antibody
      • Mediator complex subunit 14 antibody
      • Mediator of RNA polymerase II transcription subunit 14 antibody
      • RGR1 antibody
      • RGR1 homolog antibody
      • Thyroid hormone receptor-associated protein complex 170 kDa component antibody
      • Transcriptional coactivator CRSP150 antibody
      • Trap170 antibody
      • Vitamin D3 receptor-interacting protein complex 150 kDa component antibody
      see all

    Images

    References (1)

    Publishing research using ab51349? Please let us know so that we can cite the reference in this datasheet.

    ab51349 has been referenced in 1 publication.

    • Englert NA  et al. Epigenetic modification of histone 3 lysine 27: mediator subunit MED25 is required for the dissociation of polycomb repressive complex 2 from the promoter of cytochrome P450 2C9. J Biol Chem 290:2264-78 (2015). PubMed: 25391650

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