Key features and details
- Rabbit polyclonal to CRSP2 / MED14
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Product nameAnti-CRSP2 / MED14 antibody
See all CRSP2 / MED14 primary antibodies
DescriptionRabbit polyclonal to CRSP2 / MED14
SpecificityThe antibody detects endogenous levels of total CRSP2 / MED14 protein.
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human, African green monkey
Predicted to work with: Mouse
Synthetic peptide corresponding to Human CRSP2/ MED14 (internal sequence).
Database link: O60244
- Human brain tissue and COS7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
(without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/500.|
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similaritiesBelongs to the Mediator complex subunit 14 family.
- Information by UniProt
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
Immunofluorscence analysis of COS-7 (African green monkey kidney fibroblast-like cell line) cells labeling CRSP2 / MED14 with ab196624 at a 1/100 dilution.
Immunohistochemical analysis of formalin fixed and paraffin embedded human brain tissue labeling CRSP2 / MED14 with ab196624 at a 1/50 dilution. Right image treated with synthesized peptide.
ab196624 has not yet been referenced specifically in any publications.