Overview

  • Product name

    Anti-CRSP2 / MED14 antibody
    See all CRSP2 / MED14 primary antibodies
  • Description

    Rabbit polyclonal to CRSP2 / MED14
  • Host species

    Rabbit
  • Specificity

    The antibody detects endogenous levels of total CRSP2 / MED14 protein.
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, African green monkey
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human CRSP2/ MED14 (internal sequence).
    Database link: O60244

  • Positive control

    • Human brain tissue and COS7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

    (without Mg2+ and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the Mediator complex subunit 14 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator-recruited cofactor 150 kDa component antibody
    • ARC150 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 2 antibody
    • CRSP complex subunit 2 antibody
    • DRIP150 antibody
    • EXLM1 antibody
    • hRGR1 antibody
    • MED14 antibody
    • MED14_HUMAN antibody
    • Mediator complex subunit 14 antibody
    • Mediator of RNA polymerase II transcription subunit 14 antibody
    • RGR1 antibody
    • RGR1 homolog antibody
    • Thyroid hormone receptor-associated protein complex 170 kDa component antibody
    • Transcriptional coactivator CRSP150 antibody
    • Trap170 antibody
    • Vitamin D3 receptor-interacting protein complex 150 kDa component antibody
    see all

Images

  • Immunofluorscence analysis of COS7 cells labeling CRSP2 / MED14 with ab196624 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed and paraffin embedded human brain tissue labeling CRSP2 / MED14 with ab196624 at a 1/50 dilution. Right image treated with synthesized peptide.

References

ab196624 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196624.
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