Anti-CRSP2 / MED14 antibody (ab72141)
Key features and details
- Rabbit polyclonal to CRSP2 / MED14
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CRSP2 / MED14 antibody
See all CRSP2 / MED14 primary antibodies -
Description
Rabbit polyclonal to CRSP2 / MED14 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human CRSP2/ MED14.
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Positive control
- Whole cell lysate from Hela cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| All applications |
Horse
Dog
Pig
Chimpanzee
Rhesus monkey
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP |
| Notes |
|---|
WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the Mediator complex subunit 14 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9282 Human
- Omim: 300182 Human
- SwissProt: O60244 Human
- Unigene: 407604 Human
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Alternative names
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
see all
Images
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Western blot - Anti-CRSP2 / MED14 antibody (ab72141)All lanes : Anti-CRSP2 / MED14 antibody (ab72141) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 160 kDa
Observed band size: 160 kDa
Additional bands at: 120 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Various other bands are detected. -
Immunoprecipitation - Anti-CRSP2 / MED14 antibody (ab72141)Immunoprecipitation/ Western Blot of CRSP2 / MED14.
Lane 1: ab72141 at 3µg/mg whole cell lysate for IP.
Lane 2: Control IgG.
ab72141 at 1µg/ml for subsequent WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab72141 has been referenced in 1 publication.
- Ruiz A et al. Characterization of the influence of mediator complex in HIV-1 transcription. J Biol Chem 289:27665-76 (2014). Human . PubMed: 25100719