Key features and details
- Rabbit polyclonal to CRSP2 / MED14
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CRSP2 / MED14 antibody
See all CRSP2 / MED14 primary antibodies
DescriptionRabbit polyclonal to CRSP2 / MED14
Tested Applications & Species
Application Species IPHuman WBHuman
Synthetic peptide corresponding to a region between residues 1 and 50 of human CRSP2/ MED14.
- Whole cell lysate from Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similaritiesBelongs to the Mediator complex subunit 14 family.
- Information by UniProt
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
All lanes : Anti-CRSP2 / MED14 antibody (ab72141) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 160 kDa
Observed band size: 160 kDa
Additional bands at: 120 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Various other bands are detected.
Immunoprecipitation/ Western Blot of CRSP2 / MED14.
Lane 1: ab72141 at 3µg/mg whole cell lysate for IP.
Lane 2: Control IgG.
ab72141 at 1µg/ml for subsequent WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 3 seconds.
ab72141 has been referenced in 1 publication.
- Ruiz A et al. Characterization of the influence of mediator complex in HIV-1 transcription. J Biol Chem 289:27665-76 (2014). Human . PubMed: 25100719