Anti-CRSP2 / MED14 antibody (ab72141)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CRSP2 / MED14 antibody
See all CRSP2 / MED14 primary antibodies -
Description
Rabbit polyclonal to CRSP2 / MED14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human CRSP2/ MED14.
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Positive control
- Whole cell lysate from Hela cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab72141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP |
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the Mediator complex subunit 14 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9282 Human
- Omim: 300182 Human
- SwissProt: O60244 Human
- Unigene: 407604 Human
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Alternative names
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
see all
Images
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Western blot - Anti-CRSP2 / MED14 antibody (ab72141)All lanes : Anti-CRSP2 / MED14 antibody (ab72141) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 160 kDa
Observed band size: 160 kDa
Additional bands at: 120 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Various other bands are detected. -
Immunoprecipitation - Anti-CRSP2 / MED14 antibody (ab72141)Immunoprecipitation/ Western Blot of CRSP2 / MED14.
Lane 1: ab72141 at 3µg/mg whole cell lysate for IP.
Lane 2: Control IgG.
ab72141 at 1µg/ml for subsequent WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 3 seconds.
Datasheets and documents
References
This product has been referenced in:
- Ruiz A et al. Characterization of the influence of mediator complex in HIV-1 transcription. J Biol Chem 289:27665-76 (2014). Human . Read more (PubMed: 25100719) »
