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    crsp2-med14-antibody-ab72141.pdf

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Epigenetics and Nuclear Signaling Transcription Mediator Complex
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Anti-CRSP2 / MED14 antibody (ab72141)

  • Datasheet
  • SDS
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Western blot - Anti-CRSP2 / MED14 antibody (ab72141)
  • Immunoprecipitation - Anti-CRSP2 / MED14 antibody (ab72141)

Key features and details

  • Rabbit polyclonal to CRSP2 / MED14
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CRSP2 / MED14 antibody
    See all CRSP2 / MED14 primary antibodies
  • Description

    Rabbit polyclonal to CRSP2 / MED14
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human CRSP2/ MED14.

  • Positive control

    • Whole cell lysate from Hela cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Mediator Complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab72141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Horse
Dog
Pig
Chimpanzee
Rhesus monkey
Orangutan
Application Abreviews Notes
WB
IP
Notes
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the Mediator complex subunit 14 family.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession O60244 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9282 Human
      • Omim: 300182 Human
      • SwissProt: O60244 Human
      • Unigene: 407604 Human
      • Alternative names

        • Activator-recruited cofactor 150 kDa component antibody
        • ARC150 antibody
        • Cofactor required for Sp1 transcriptional activation subunit 2 antibody
        • CRSP complex subunit 2 antibody
        • DRIP150 antibody
        • EXLM1 antibody
        • hRGR1 antibody
        • MED14 antibody
        • MED14_HUMAN antibody
        • Mediator complex subunit 14 antibody
        • Mediator of RNA polymerase II transcription subunit 14 antibody
        • RGR1 antibody
        • RGR1 homolog antibody
        • Thyroid hormone receptor-associated protein complex 170 kDa component antibody
        • Transcriptional coactivator CRSP150 antibody
        • Trap170 antibody
        • Vitamin D3 receptor-interacting protein complex 150 kDa component antibody
        see all

      Images

      • Western blot - Anti-CRSP2 / MED14 antibody (ab72141)
        Western blot - Anti-CRSP2 / MED14 antibody (ab72141)
        All lanes : Anti-CRSP2 / MED14 antibody (ab72141) at 0.04 µg/ml

        Lane 1 : Whole cell lysate from Hela cells at 50 µg
        Lane 2 : Whole cell lysate from Hela cells at 15 µg
        Lane 3 : Whole cell lysate from Hela cells at 5 µg

        Predicted band size: 160 kDa
        Observed band size: 160 kDa
        Additional bands at: 120 kDa, 71 kDa. We are unsure as to the identity of these extra bands.



        Various other bands are detected.
      • Immunoprecipitation - Anti-CRSP2 / MED14 antibody (ab72141)
        Immunoprecipitation - Anti-CRSP2 / MED14 antibody (ab72141)
        Immunoprecipitation/ Western Blot of CRSP2 / MED14.
        Lane 1: ab72141 at 3µg/mg whole cell lysate for IP.
        Lane 2: Control IgG.

        ab72141 at 1µg/ml for subsequent WB.
        Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
        Detection:Chemiluminescence with an exposure time of 3 seconds.

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab72141? Please let us know so that we can cite the reference in this datasheet.

      ab72141 has been referenced in 1 publication.

      • Ruiz A  et al. Characterization of the influence of mediator complex in HIV-1 transcription. J Biol Chem 289:27665-76 (2014). Human . PubMed: 25100719

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