Product nameAnti-CRSP2 / MED14 antibody
See all CRSP2 / MED14 primary antibodies
DescriptionRabbit polyclonal to CRSP2 / MED14
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide corresponding to a region between residues 1 and 50 of human CRSP2/ MED14.
- Whole cell lysate from Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similaritiesBelongs to the Mediator complex subunit 14 family.
- Information by UniProt
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
All lanes : Anti-CRSP2 / MED14 antibody (ab72141) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 160 kDa
Observed band size: 160 kDa
Additional bands at: 120 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Various other bands are detected.
Immunoprecipitation/ Western Blot of CRSP2 / MED14.
Lane 1: ab72141 at 3µg/mg whole cell lysate for IP.
Lane 2: Control IgG.
ab72141 at 1µg/ml for subsequent WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 3 seconds.